摘要
目的:探究肺结核患者外周血中CD8^+T细胞的水平表达及其对结核分枝杆菌耐药的相关性。方法:采用FACS Calibur流式细胞仪分别对药物全敏感(DS)肺结核患者、单耐药(SDR)肺结核患者、耐多药(MDR)肺结核患者、多耐药(MDRS)肺结核患者和泛耐药(PDR)肺结核患者外周血CD8^+T细胞及其亚群水平的表达进行检测,并采用相应的统计学方法进行比较分析。结果:SDR组患者CD8^+T细胞水平的表达显著高于健康组和PDR组(P<0.05),但在其他组间的比较中均无统计学意义(P>0.05);进一步分析CD8^+T细胞亚群水平在各组肺结核患者和健康人外周血中的表达,CD8^+CD28^+T细胞在健康组中的表达显著高于SDR组和PDR组(P<0.05);分析CD8^+CD28^+T细胞在各组肺结核患者中的表达,发现在DS组中的表达显著高于SDR组(P<0.05);CD8^+CD28-T细胞在SDR组的表达显著高于MDR组、MDRS组及PDR组(P<0.05)。结论:不同耐药性肺结核患者外周血CD8^+T细胞的表达水平存在一定差异,CD8^+T细胞及其亚群的检测有助于进一步阐明机体免疫状态与结核分枝杆菌耐药之间的相互关系,从而为结核病患者的免疫干预以及免疫治疗提供重要的理论依据。
Objective:To investigate the expression of CD8^+T cells level in peripheral blood of patients with tuberculosis(TB)and its correlation with Mycobacterium tuberculosis drug resistance.Methods:FACSCalibur flow cytometry was used to detect the expression of CD8^+T cells and their subsets level in peripheral blood of patients with drug sensitive(DS)tuberculosis,single drug resistant(SDR)tuberculosis,multidrug resistant(MDR)tuberculosis,multidrug resistants(MDRS)tuberculosis and pan-drug resistant(PDR)tuberculosis,and the corresponding statistical methods were used for comparative analysis.Results:The expression of CD8^+T cells level in SDR group was significantly higher than that in healthy group and PDR group(P<0.05),but there was no statistical significance in the comparison between other groups(P>0.05).Further analysis of CD8^+T cells subgroup level in tuberculosis patients and healthy people in the peripheral blood,CD8^+CD28^+T cells in healthy group expression is significantly higher than that of the SDR and PDR group(P<0.05).The expression of CD8^+CD28^+T cells in tuberculosis patients of each group was analyzed,and it was found that the expression of CD8^+CD28^+T cells in the DS group were significantly higher than those in the SDR group(P<0.05).The expression of CD8^+CD28-T cells in SDR group was significantly higher than that in MDR group,MDRS group and PDR group(P<0.05).Conclusion:The expression level of CD8^+T cells in peripheral blood of patients with different drug resistance tuberculosis is different to some extent.The detection of CD8^+T cells and their subsets is helpful to further elucidate the correlation between the immune status of the body and mycobacterium tuberculosis drug resistance,thus providing an important theoretical basis for the immune intervention and immunotherapy of tuberculosis patients.
作者
薛婧
宋华峰
赵静
陈慧
胥萍
XUE Jing;SONG Hua-feng;CHENG Hui;ZHAO Jing;XU Ping(THe No.5 People's Hospital of Suzhou,Suzhou Jiangsu 215123,China)
出处
《抗感染药学》
2019年第8期1309-1312,共4页
Anti-infection Pharmacy
基金
江苏省科技基金项目(编号:BK20161230)
苏州市青年科技基金项目(编号:KJXW2016041)
