摘要
目的探讨外周血淋巴细胞亚群的比例变化在结核病的临床意义。方法采用流式细胞仪检测继发性肺结核患者93例(其中初治肺结核49例,复治耐药肺结核44例),急性血行播散性肺结核29例患者的外周血CD3+、CD4+、CD8+淋巴细胞亚群及CD4+/CD8+比值。结果急性血行播散性肺结核患者组外周血CD8+T细胞比例明显增高[(34.64±5.45)%],而CD3+[(63.29±4.16)%]、CD4+[(27.81±6.17)%]及CD4+/CD8+[(0.81±0.42)]则明显降低,与初治肺结核组及复治耐药肺结核组比较,差异均有统计学意义(P<0.05)。初治肺结核与复治耐药肺结核组间CD3+、CD4+、CD8+及CD4+/CD8+比较,差异无统计学意义。继发性肺结核患者病变范围广泛者(病灶≥3个肺野)的CD3+T细胞比例为(65.37±9.9)%,明显低于病变范围小者[(74.49±1.07)%],差异有统计学意义(P<0.05)。结论 T淋巴细胞亚群在结核病患者外周血中分布失衡,可能与疾病的发展相关。监测结核病患者T淋巴细胞亚群水平,对评价患者的细胞免疫功能及对疾病的免疫治疗均有重要的指导意义。
Objective To observe the clinical significance of lymphocyte subsets of peripheral blood in pulmonary tuberculosis （PTB） patients. Methods A total of 93 cases of secondary PTB and 29 cases of acute hemotogenous dissemi- nated PTB were enrolled in the study. The CD3＋ ,CD4＋ ,CD8＋ and CD4＋/CD8＋ lymphocytes of peripheral blood in PTB pa- tients were detected by flow cytometry. Results Compared with secondary PTB, CD8＋ lymphocytes [（34.64±5.45）%] in- creased significantly, while CD3＋[（63.29±4.16）%], CD4＋ lymphocytes [（27.81±6.17）%] and CD4＋/CD8＋ ratios （0.81±0.42） decreased in acute hemotogenous disseminated PTB. There were statistically significant differences （P 〈 0.05） between the two groups. There were no significant differences of CD3＋,CD4＋,CD8＋ lymphoeytes and CD4＋/CD8＋ ratios in untreated PTB and drug-resistance retreatment PTB. CD3＋ lymphocytes decreased significantly [（65.37±9.9）%] in extensive lesions group. Conclusion The disbalanee of peripheral blood lymphocyte subsets in PTB patients may contribute to the development of PTB. Analysis of T lymphocyte subsets is helpful to evaluate the immune status and apply immunotherapy for the patients.
出处
《北京医学》
CAS
2013年第12期993-995,共3页
Beijing Medical Journal
基金
"十二五"国家科技重大专项(2012ZX09303-002)
关键词
肺结核
T淋巴细胞亚群
流式细胞术
Lung tuberculosis T Lymphocyte subsets Flow cytometry
