摘要
在构建了含毛细胞白血病相关的结构性倒位inv(5)(p13.1q13.3)的细胞系后,为了确定该新建细胞系在建株过程中其倒位断裂点关键区遗传物质是否发生改变,以生物素或地高辛标记的cCI5-216和cCI5-267黏粒DNA为探针,进行染色体中期、间期和DNA纤维3种双色荧光原位杂交的分析。结果表明:该新建细胞系的3种双色荧光原位杂交结果,均与该细胞系的原代细胞的完全相同,证实了该细胞系倒位断裂点关键区的遗传物质结构未发生改变。该细胞系是揭示毛细胞白血病发病的分子机理的重要研究材料。
To determine whether genetic material in 5q13.3 breakpoint region changed in the course of establishing the cell line with a constitutional chromosomal inversion inv(5)(p13.1q13.3) associated with hairy cell leukemia,double color fluorescence in situ hybridization (FISH) on metaphase,interphase and DNA fibers were performed in cells of the cell line with the cosmids cCI5-216 and cCI5-267 DNA probes labeled by either biotinylate or digoxigenin.It showed that the cell line gave the same results as those of the original cells,which indicated that no change of the genetic material at 5q13.3 breakpoint region occurred.The cell line is valuable to reveal the molecular pathogenesis of hairy cell leukemia.
出处
《遗传》
CAS
CSCD
北大核心
2003年第1期1-4,共4页
Hereditas（Beijing)
基金
国家自然科学基金资助项目(30170479)
湖南省特聘教授基金资助(25000613)
教育部留学归国启动基金资助
