摘要
目的通过检测细胞周期素D_1在正常胃组织及胃癌组织中的表达,探讨其与胃癌分化程度及5年生存率间的关系。方法应用流式细胞术(FCM)对13例正常胃组织及27例胃癌组织细胞进行细胞周期素D_1含量的定量检测。结果细胞周期素D_1在正常组的表达均为阴性,77.8%(21/27)的胃癌组织细胞周期素D_1呈阳性表达,其表达量(FI值)和阳性表达率与胃癌组织分化程度及5年生存率有明显相关性(P﹤0.01)。结论细胞周期素D_1过表达与胃癌组织的分化程度及预后有明显相关性,其高表达与胃癌的发生、发展有密切关系,检测cyclin D_1蛋白有助于胃癌恶性程度及预后的判断。
Objective To detect the expression of cyclinDi proteins in normal gastric tissue and gastric cancer, and to study correlation with differentiation of gastric cancer. Methods By using Flow cytometry to detect the expression of cyclinDi proteins in normal gastric tissue and gastric cancer tissue. Results The expression of cyclinDi was negative in normal gastric tissue, 21of 27 （77.8%） gastric cancer tissure were positive, cyclinDl positive expression rate and content were correlation with differentiation of gastric cancer and rate of 5 year survival （P 〈 0.01）. Conclusion The overexpression of cyclinDl proteins is correlated with differentiation of gastric cancer and progenosis. The overexpression of cyclinD1 proteins is closely associated with occurrence and development of gastric cancer. To detect cyclinDi proteins may be useful for evaluating gastric cancer differentiantion and progenosis.
作者
张宏
ZHANG Hong(Affiliated hospital, Inner mongolia medical university, Huhehaote010050, China)
出处
《疾病监测与控制》
2017年第2期116-117,共2页
Journal of Diseases Monitor and Control
