摘要
①目的 检测基质金属蛋白酶组织抑制物 1(TIMP 1)基因在胃癌及其癌旁组织中的表达 ,以探讨TIMP 1在胃癌发生发展中的作用。②方法 应用逆转录 聚合酶链反应 (RT PCR)检测胃癌及相应癌旁组织中TIMP 1基因的表达。③结果 30例胃癌组织中有 16例表达TIMP 1基因 ,阳性率为 5 3.3% ,30例癌旁组织中有14例表达TIMP 1基因 ,阳性率为 4 6 .7% ,TIMP 1基因在胃癌和癌旁组织中的表达无显著性差异 (χ2 =0 .2 6 7,P >0 .0 5 )。④结论 TIMP 1是一种肿瘤生长、侵袭、转移双向调节因子。
Objective\ To study the expression of TIMP 1 gene in gastric carcinoma and its adjacent tissues, so as to find out the role of TIMP 1 gene in the development of the carcinoma.\ Methods\ TIMP 1 gene expression in cancer tissue and its adjacent area was detected by reverse transcriptase polymerase chain reaction (RT PCR). \ Results\ Positive expression of TIMP 1 gene was noted in 16 (53.3%) of the 30 gastric carcinoma specimens and 14(46.7%) in the adjacent tissue. No significant difference was found between the expression of TIMP 1 gene in the carcinoma and the adjacent tissue ( χ 2=0.267, P >0.05).\ Conclusion\ TIMP 1 is a bi directional regulating factor attributing to the development, invasion and metastasis of the tumor.
出处
《青岛大学医学院学报》
CAS
2003年第1期20-22,共3页
Acta Academiae Medicinae Qingdao Universitatis
关键词
胃肿瘤
金属内肽酶类
金属蛋白酶类组织抑制剂
基因表达
聚合酶链反应
gastric neoplasms
metalloendopeptidases
tissue inhibitor of metalloproteinases
gene expression
polymerase chain reaction
