摘要
目的 观察蘑菇多糖对小鼠腹腔巨噬细胞免疫调节作用及对体外培养的肿瘤细胞作用的影响。方法 采用比色法测定经蘑菇多糖处理的巨噬细胞内LDH的活性。MTT法观察蘑菇多糖与巨噬细胞的共同培养上清对HL - 60细胞及K56 2 细胞的抑制率。结果 (1)蘑菇多糖明显激活小鼠腹腔巨噬细胞 ,显著提高巨噬细胞的LDH活性 ;(2 )蘑菇多糖作用下的巨噬细胞上清液可显著抑制HL - 60细胞及K56 2 细胞的增殖。
Objective To observe the immunomodulatory effect of agaricus proteoglucan on the peritoneal macrophage of mice and its influence to the cultured tumor cells in vitro. Methos Colormetry was used to assay the activity of the LDH.The proliferation ihhibition effects of agaricus proteoglucan on HL-60 cell or K 562 cell were observed by MTT colorimetry.Results (1) Peritoneal macrophage was significantly activated by agaricus proteoglucan, and there was an obvious increase in the activity of LDH; (2) The supernatant of peritoneal macrophage treated with agaricus proteoglucan could inhibit the proliferation of HL-60 and K 562 . Conclusion The anti-tumor effect of agaricus proteoglucan may be correlated to its activation of peritoneal macrophage.
出处
《苏州大学学报（医学版）》
CAS
2003年第1期10-12,共3页
Suzhou University Journal of Medical Science
