摘要
目的 研究藻毒素对c fos、c jun表达及细胞周期的影响 ,探讨其可能的致癌机制。方法 将经色谱柱纯化的微囊藻毒素样品加入到SHE细胞培养体系中 ,用免疫组化方法分别于第 1、3、6h观察细胞c fos和c jun蛋白表达 ,并用流式细胞仪分别检测细胞在第 6、1 2、2 4h不同时相的周期改变情况。结果 微囊藻毒素可以诱导c fos和c jun持续高表达 ,6h后增高至 5～ 6倍 ,具有明显的剂量反应关系 ;同时 ,藻毒素尚能介导G0 /G1 期细胞进入分裂状态 ,S、G2 /M期细胞比例明显增加 ,2 4h后有 44 9%细胞进入S期。结论 诱导细胞c jun和c
Objectives To investigate the effects of microcystins on cell cycle and expressions of c fos and c jun, and explore the potential carcinogenic mechanisms of Microcystins Methods Microcystic cyanobacteria extraction (MCE) purified by Sep Pak C 18 cartridge was added into the media and co incubated with SHE cell for various periods Immunohistochemistry assay was applied to detect the expressions of c fos and c jun at 1?3?6 hr time point, and cell cycle at 6?12?24 hr point were analyzed by flow cytometry respectively Results Sustained up regulated expression of c fos and c jun were induced by MCE during the experimental period, and 5-?6 folds increased expression were observed at 6 hour point after treatment As much as 44 8 per cent of cells were induced to entry S phase from resting G 0/G 1 Conclusion Up regulating the expression of transcript factor such as c fos and c jun thus to induce cell abnormal proliferation may be the potential carcinogenic mechanisms of microcystins
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2003年第1期23-25,共3页
Chinese Journal of Preventive Medicine
基金
国家自然基金资助 ( 39970 6 2 8)
