摘要
目的 研究北沙参农家品种的鉴定。方法 利用蛋白质与过氧化物酶 (POD)同工酶的聚丙烯酰胺凝胶电泳 (PAGE)指纹图谱分析、鉴别北沙参农家品种。结果 3种样品的蛋白质PAGE指纹图谱具有一定差异 ,POD同工酶指纹图谱具有显著性差异。结论 PAGE指纹图谱快速、灵敏、技术简单 。
OBJECTIVE: To study the identification of Radix Glehniae's varieties. METHODS: The polyacrylamide gel electrophoresis (PAGE) fingerprints of protein and peroxidase (POD) isozyme was analyzed to identify the three varieties of Radix Glehniae. RESULTS: There was some difference to a certain extent on their protein PAGE fingerprints. Their POD isozyme PAGE fingerprints were significantly different. CONCLUSION: PAGE fingerprint has many advantages such as fast, high sensitivity and simple operation. It can be used for identification and quality evaluation of cultivated varieties of Radix Glehniae.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2003年第3期171-173,共3页
Chinese Pharmaceutical Journal
基金
国家科技部项目"金银花
北沙参
瓜蒌的规范化种植研究"(编号 :99-92 9-0 1-2 0 )
关键词
北沙参
PAGE
指纹图谱
Electrophoresis
Polyacrylates
Sensitivity analysis
