摘要
目的:观察当归总多糖(ASP)对正常及泼尼松龙(PSL)所致肝损伤小鼠肝脏药物代谢酶活性的影响。方法:PSL皮下注射15mg·kg-1,连续5d造成小鼠肝损伤。酶学测定Ⅱ相酶谷胱甘肽相关酶活性;测定细胞色素P450(CYP)含量、NADPH-细胞色素C还原酶、氨基比林N-脱甲基酶(AMD)及苯胺羟化酶(ANH)活性。结果:ASPig使正常小鼠肝微粒体及线粒体谷胱甘肽S-转移酶(GST)活性升高,肝线粒体谷胱甘肽(GSH)含量、谷胱甘肽还原酶活性无明显改变,对肝微粒体细胞色素P450酶系统有诱导作用。大剂量PSL使小鼠血清ALT明显升高,肝线粒体GSH含量明显下降,上述各项酶活性升高。ASP对PSL所致酶活性及GSH含量改变有恢复作用。结论:ASP具有调节肝脏药物代谢酶活性作用。
Objective: To study the effects of Angelica sinensis Polysaccharides (ASP) on the hepatic drug metabolism enzymes activities in normal mice and those prednisolone (PSL)-induced liver injury. Method: The activities of phase Ⅱ enzymes (GSH-related enzymes) and cytochrome P450 enzymes were measured by biochemical method. Result: ASP increased the activities of glu-tathione S-transferase in liver microsomes and mitochondria. The cytochrome P450 content, NADPH-cytochrome c reductase, aminopyrine N-demethylase, and aniline hydroxylase activities in liver microsomes were also increased. PSL significantly increased serum ALT levels, and decreased the liver mitochondrial glutathione content. At the same time, other enzymes activities were all increased. When mice were treated with ASP 2.0g·kg-1, the PSL-induced changes on cytochrome P450 enzymes, glutathione S-transferase, and GSH content were restored. Conclusion: ASP can modulate the activities of drug metabolism enzymes.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2003年第2期149-152,共4页
China Journal of Chinese Materia Medica
基金
湖北省教委项目(G9501)
关键词
当归多糖
泼尼松龙
微粒体
线粒体
肝脏药物代谢酶
Angelica sinensis Polysaccharides
prednisolone
microsome
mitochondria
hepatic drug metabolism enzymes
