摘要
目的 测定感染细粒棘球蚴 (E.g)绵羊诱发过敏性休克期间特异性 Ig G、Ig G1和 Ig E抗体水平。了解抗原B对人工感染 E.g绵羊 Ig G抗体的反应性。 方法 从绵羊 E.g囊液中制备抗原 B和 E.g囊液粗制抗原 ,EL ISA测定感染 E.g绵羊诱发休克期间特异性 Ig G、Ig G1和 Ig E抗体的动态变化。 结果 感染 E.g绵羊 6个月 ,特异性 Ig G、Ig G1和 Ig E抗体水平较正常绵羊显著升高 ;诱发休克后特异性 Ig E抗体水平显著下降 ,尤其因休克致死的绵羊下降更为明显 ;Ig G及 Ig G1抗体的衰减时间不同 ,趋势各不相同 ;抗原 B和 E.g囊液粗制抗原与血清 Ig G抗体反应阳性率分别为 91%、32 %。 结论 特异性 Ig E是导致棘球蚴病所致过敏性休克的主要抗体 ,而 Ig G和 Ig G1抗体也起着重要作用。抗原 B与感染 E.g绵羊 Ig G抗体的血清反应性较好 ,可作为一种血清免疫学诊断方法监测绵羊感染 E.g的状况。
Objective To investigate the change of specific antibodies IgG, IgG1 subclass and IgE in sheep infected with Echinococcus granulosus(E.g) during anaphylactic shock, and to observe antigen B reactivity against IgG antibody in E.g\|infected sheep. \ Methods\ Antigen B and crude antigen were prepared with E.g cyst fluid (EgCF) from infected sheep. The enzyme\|linked immunosorbent assay(ELISA) was used for detecting the level of specific IgG, IgG1 and IgE during anaphylactic shock in sheep induced by E.g. \ Results \ The level of specific IgG, IgG1 and IgE was significantly higher in the infected sheep after 6 months than that of the uninfected control group (P<0\^01). The IgE level decreased rapidly after anaphylactic shock induced, especially when the sheep was dying. Following an antigen challenge the sheep showed a general decrease in total IgG and IgG1 subclass. The total IgG showed a slight change at the beginning,followed by a decrease 1 h after challenge. The decrease of IgG1 subclass was more significant than the total IgG in 40 min after challenge injection. The positive rate was 91% for antigen B and 32% for crude antigen of EgCF against IgG antibody in E.g\|infected sheep. \ Conclusion \ The specific IgE plays a major role in the anaphylactic shock, while IgG and IgG1 \{antibodies\} are also important. Antigen B derived from sheep E.g cyst fluid appears to be useful in serodiagnosis of and \{monitoring\} on the infection status in sheep.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2003年第1期42-45,共4页
Chinese Journal of Parasitology and Parasitic Diseases
基金
国家自然科学基金 (No.39860 0 78)~~
