摘要
目的探讨抗中性粒细胞胞浆抗体(ANCA)在系统性红斑狼疮(SLE)患者中检测的临床意义。方法通过间接免疫荧光法(IIF)检测57例SLE患者血清中的ANCA,对ANCA阳性血清采用免疫斑点法检测抗髓过氧化物酶(MPO)和蛋白水解酶3(PR3);回顾性分析SLE临床表现和实验室检查结果,SLE患者疾病活动程度性评判以SLEDAI分组(SLEDAI≥10为活动组、【10为非活动组),分析ANCA与其相关性。结果 SLE患者中ANCA阳性率为22.8%,其中核周型(pANCA)阳性12例,阳性率为21.1%,胞浆型(cANCA)1例,阳性率为1.7%,靶抗原均为非MPO、PR3;ANCA阳性组与ANCA阴性组SLE活动性存在显著性差异(P【0.05),ANCA阳性组患者的补体C3下降、血沉(ESR)增高、抗dsDNA抗体阳性以及皮肤血管炎和肾脏损伤与ANCA阴性组比较,差异有统计学意义(P【0.05)。结论 ANCA在SLE中有一定阳性检出率,对疾病活动性判断有一定临床价值。
Objective To explore the diagnostic value of anti-neutrophil cytoplasmic antibody(ANCA) detection in patients with systemic lupus erythematosus(SLE).Methods The expression of serum ANCA in 57 samples of patients with SLE were tested by indirect immumofluorescence(IIF),and myeloperoxidase(MPO) and protease 3(PR3) were detected by immune spots method when serum ANCA were positive.We reviewed clinical manifestations and laboratory test results of SLE.We also divided patients into two groups to follow SLE disease activity index(SLEDAI)(SLEDAI≥10 as the disease activity group,SLEDAI<10 as inactivity group) and analysed their relevance.Results The positive rate of ANCA in SLE was 22.8%,in which peripheral-ANCA(PANCA)were positive in 12 patients and cytoplasmic-ANCA(CANCA) was positive in one case.The positive rate of PANCA was 21.1%.There was a remarkable difference(P<0.05) between ANCA-positive SLE patients and ANCA-negative group within the indexes of SLEDAI.The ANCA-positive group had higher titers of anti-dsDNA antibody,lower levels of complements(c3),higher erythrocyte sedimentation rate,skin vasculitis and kidney damage compared to ANCA negative patients(P<0.05).Conclusion The positive rate of ANCA in SLE patients is of value for detection of SLE activity.
出处
《浙江检验医学》
2012年第1期9-11,共3页
Zhejiang Journal of Laboratory Medicine
关键词
系统性红斑狼疮
抗中性粒细胞胞浆抗体
Lupus erythematosus
Systemic
Antineutrophil cytoplasmic antibody
