摘要
目的探讨miR-145在胃癌中的表达及其临床意义。方法选取2011年2月至2012年6月江苏省中医院就诊的67例胃癌患者,采用荧光实时定量聚合酶链反应(PCR)技术检测胃癌患者miR-145的表达情况,取肿瘤边缘外5cm处的非癌胃黏膜作为对照,比较miR-145在正常组织及胃癌组织中的表达差异及与胃癌临床病理参数的相关性。结果 54例(80.60%)胃癌患者胃癌组织miR-145表达明显低于正常组织,miR-145的表达在不同性别、年龄及TNM分期患者间的差异无统计学意义(P>0.05),但在不同组织学分级及淋巴结转移情况患者间的差异有统计学意义(P<0.01)。结论 miR-145在胃癌中呈低表达,可能与胃癌的发生、发展密切相关,可成为胃癌诊断及预后判断的指标。
Objective To investigate the expression status and clinical significance of miR-145 in gastric carci-noma .Methods miR-145 levels in 67 patients with gastric carcinoma were detected by using quantitative real -time fluorescent polymerase chain reaction .The relationship between miR-145 expression and clinicopathological factors of gastric carcinoma was analyzed .Results 54 patients were with low expression level of miR-145 in the 67 patients ,ac-counting for 80 .60% ,and the expression level of miR-145 in gastric carcinoma tissue samples was significantly lower than normal tissues .miR-145 expression level was not associated significantly with age and gender of patients ,and TNM staging (P>0 .05) ,but closely correlated with histology grading and lymphatic metastasis (P<0 .01) .Con-clusion The expression level of miR-145 might be low in patients with gastric carcinoma ,which could be correlated with the pathogenesis and development of diseases ,and could be the indicator of diagnosis and prognosis of gastric carcinoma .
出处
《检验医学与临床》
CAS
2014年第7期896-897,共2页
Laboratory Medicine and Clinic
关键词
MIR-145
胃癌
实时定量聚合酶链反应
miR-145
gastric carcinoma
quantitative real-ime polymerase chain reaction
