摘要
目的研究门静脉奇静脉断流术(下称断流术)对胃粘膜细胞增殖能力的影响,并试用降钙素基因相关肽(calcitonin generalated peptide,CGRP)保护术后胃粘膜。方法 Wistar 大鼠分正常对照组8只,肝硬变合并门静脉高压性胃病(portal hypertensive gastropathy,PHG)模型对照组12只,断流手术组12只,断流术加 CGRP 保护组12只,用放射自显影技术测定各组胃粘膜细胞增殖参数。结果肝硬变 PHG 大鼠断流术后胃粘膜细胞 DNA 合成期、细胞周期较正常及模型组延长(P<0.01),标记指数相应降低(P<0.01)。各参数变化主要表现在胃体部。应用 CGRP 后 DNA 合成期、细胞周期缩短,标记率上升(P<0.01)。结论断流术削弱了胃粘膜细胞增殖能力,可能是易发生术后 PHG 胃出血的因素之一,CGRP改善了粘膜细胞增殖受抑状态,可保护胃粘膜,减少出血。
Objective To investigate the cytokinetic mechanism behind changes of gastric mucosal epithelia prolifer- ation after pertaazygous disconnection and the protection of calcitonin generelated peptide(CGRP) on postopera- tive gastric mucosa.Methods Wistar rats were divided into four groups:normal group(n=8),model group with cirrhosis and portal hypertensive gastropathy(PHG)(n=12),disconnective group(n=12)and post-discon- nection plus CGRP group(n=12).Gastric mucesal cellular proliferative parameters from the above groups were measured by the radioscintigraphy.Results Compared with the normal and the model rats.Ts and Tc stages of gastric mucosal cells in the postoperative rats were significantly prolonged(P<0.01),an apparent lowering of labelling index(LI)appeared(P<0.01).The change was gnifant in gastric body,not in gastric antral por- tion.After the postoperative rats were treated by CGRP Tc.stages and LI recovered (P<0.01).Conclusions Disconnective operation could weaken gastric mucosal lular proliferative ability,this could be one of the re- bleeding factors.CGRP showed a considerable protection for the gastric mucosa against postoperative rebleeding.
出处
《中华普通外科杂志》
CSCD
1998年第2期117-119,共3页
Chinese Journal of General Surgery
