摘要
目的 :研究亚低温对缺氧缺血性脑损伤的远期保护作用。方法 :Wistar 7d龄新生大鼠 ,随机分为缺氧缺血后 2 4h亚低温干预组、48h亚低温干预组、72h亚低温干预组、常温 (37℃ )恢复组、正常对照组 (假结扎组 )。亚低温组每组 1 2只动物 ,各取 2只作为探针动物。其余每组 1 0只动物。制造缺氧缺血性脑损伤模型 ,于亚低温干预后 35d ,用迷宫实验检测其学习和记忆能力 ,然后断头处死 ,制作脑组织病理切片 ,作微管关联蛋白 2免疫组织化学染色 ,染色结果经计算机图像分析系统处理 ,计算脑梗死面积。结果 :亚低温干预各组第 1d和第 2d迷宫实验达标所需反应次数明显少于常温恢复组 (P <0 .0 5) ,正确反应率明显高于常温恢复组 (P <0 .0 5)。随着亚低温干预持续时间的延长 ,所需反应次数逐渐减少 ,正确反应率逐渐提高。亚低温干预各组脑梗死面积比常温恢复组显著减少 (P <0 .0 5)。且随着亚低温干预持续时间的延长 ,梗死面积逐渐减少。结论 :亚低温对新生大鼠缺氧缺血性脑损伤有远期保护作用 ,且随着亚低温干预持续时间的延长 。
Aim:To evaluate the long term effects of hypothermia on neonatal rat hypoxic ischemic brain damage. Methods: Seven day old newborn Wistar rats after hypoxic ischemic treatment were randomized into 5 groups:24 h hypothermia group, 48 h hypothermia group,72 h hypothermia group, normothermic group(37 ℃),and control group. There were 12 rat pups in each hypothermia group and 10 rat pups in the other groups. Two rats were randomly selected as probe animals in each hypothermia group for monitoring temperature. After 35 d, pups of each group were trained and tested using maze test for learning and memory ability, then sacrificed and made brain slices for histology examination of MAP2 immunohistochemistry. Results: Hypothermia groups need fewer reaction time to reach the standard and had higher correct reaction rate on the first day and second day in the maze test compared with normothermic group ( P <0.05). The longer was the hypothermia treatment, the fewer times need, and the higher correct reaction rate got. The infarct area significantly decreased in all the hypothermia groups compared with that in the normothermic group ( P <0.05). The longer was the hypothermia treatment, the smaller infarct area got.Conclusion: Hypothermia has long term protective effects on neonatal rat hypoxic ischemic brain damage. The longer is the hypothermia treatment, the better protective effects get.
出处
《郑州大学学报(医学版)》
CAS
北大核心
2003年第2期215-217,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省医学创新人才工程项目2 0 0 0 84 0 0 1
河南省杰出青年基金 2 0 0 0 6
关键词
亚低温
新生大鼠
缺氧缺血性脑损伤
远期保护
neonatal rat
hypoxic ischemia brain damage
microtubule associated protein 2
maze test
hypothermia