摘要
为探讨鹀科鸟类间的亲缘关系和系统发育特点,应用AFLP分子标记技术,对采自中国吉林地区的鹀科17种鸟类67只个体进行了遗传多态性研究。利用9对引物,共扩增出410条带,其中351个多态条带。AFLP聚类分析结果表明:17种鹀科鸟类聚为两大进化分支,其中除栗耳鹀外的所有鹀属鸟类聚为A分支,而雪鹀、铁爪鹀和栗耳鹀聚为B分支。当相似性系数为0. 75时,17种鹀科鸟类可分为8个类群:第1类为栗斑腹鹀和三道眉草鹀;第2类为白头鹀;第3类为白眉鹀、灰头鹀、黄眉鹀、黄胸鹀、田鹀、小鹀和栗鹀;第4类为芦鹀、苇鹀和红颈苇鹀;第5类为黄喉鹀;第6类为雪鹀;第7类为铁爪鹀;第8类为栗耳鹀。研究结果表明,各类群内不同种鹀间存在较近的亲缘关系。
To discuss genetic relationship and phylogenesis among buntings,we used amplified fragment length polymorphism ( AFLP) molecular marker technique to study the genetic diversity of 67 individuals from 17 species collected from Jilin province. Nine primers were selected to conduct AFLP-PCR,and 410 DNA bands were obtained including 351 polymorphic bands. According to the clustering results of AFLP,17 bunting species were divided into two distinct clades: all birds from Emberiza cluster A clade except E. fucata,and the B clade included Calarius lapponicas,Plectrophenax nivalos and E. fucata. When the similarity coefficient was 0. 75,17 bunting species could be divided into 8 groups. The first group consisted of E. jankowskii and E. cioides;the second group consisted of E. leucocephalos;the third group consisted of E. tristrami,E. spodocephala,E. chrysophrys, E. aureola,E. rustica,E. pusilla and E. rutile;the fourth group consisted of E. schoeniclus, E. pallasi and E. yessoensis;the fifth group consisted of E. elegans;the sixth group consisted of P. nivalos;the seventh group consisted of C. lapponicas;the eighth group consisted of E. fucata. These results indicated that different bunting species in the same group has close genetic relation ship.
作者
张立世
李时
王海涛
姜云垒
ZHANG Lishi;LI Shi;WANG Haitao;JIANG Yunlei(College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;School of Life Sciences,Northeast Normal University,Changchun 130024,China)
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2018年第6期748-753,共6页
Journal of Jilin Agricultural University
基金
国家自然科学基金项目(31601856
31270471)
吉林省自然科学基金项目(20101558)
