摘要
以小型猪(巴马香猪46头)和大型猪(长白猪、大白猪、杜洛克猪,共198头)为试验对象,采用PCR产物直接测序法对胰岛素样生长因子1受体(IGF1R)外显子进行SNPs筛查,根据测序结果确定每个SNP位点的基因型,并通过Haploview软件对筛选到的SNPs进行连锁不平衡及单倍型分析。结果表明:在不同体型猪IGF1R基因外显子1~21上共筛选到9处同义SNPs位点,为G263C(rs338724264)、T17G(rs325909655)、C145T (rs337838116)、 T40C (rs326728191)、 C104T (rs319719983)、 C170T (rs338444971)、 C60T(rs327750836)、C126T(rs327750836)和C82T(rs339449791)。这9个SNPs在巴马香猪和大型猪中均为纯合子突变,且呈现完全连锁不平衡。此研究明确了不同体型猪IGF1R基因外显子区SNPs的基因型及由这些SNPs形成的优势单倍型,可为探究不同猪种生长发育过程中体尺存在差异的原因提供新数据。
A total of 46 miniature pigs ( Bama miniature pigs) and 198 large pigs ( Landrace,Yorkshire and Duroc pigs) were used as experimental subjects. SNPs in the exon region of the insulinlike growth factor 1 receptor ( IGF1R) were determined by direct sequencing of PCR products. The genotype of each SNP locus was determined based on the sequencing results,and the linkage disequilibrium and haplotype analysis of the selected SNPs were performed by Haploview software. The experimental results show that there are 9 synonymous SNPs in exon 1-21 of IGF1R gene,which are as follows: G263C ( rs338724264 ) , T17G ( rs325909655 ) , C145T ( rs337838116 ) , T40C ( rs326728191) ,C104T ( rs319719983) ,C170T( rs338444971) ,C60T( rs327750836) ,C126T ( rs327750836) and C82T( rs339449791) . The 9 SNPs showed complete linkage disequilibrium and formed a haplotype ( P<0. 0001) in both Bama miniature pigs and large pigs. This study clarified the genotypes of SNPs in the exon regions of IGF1R genes of different body type pigs and the dominant haplotypes formed by these SNPs providing new data for clarifying the reasons for the difference in body size during the growth and development of different pig breeds.
作者
李常红
张明国
胡玮宜
魏文珍
杨睿
郝林琳
LI Changhong;ZHANG Mingguo;HU Weiyi;WEI Wenzhen;YANG Rui;HAO Linlin(College of Life Science,Baicheng Normal University,Baicheng 137000,China;Dunhua Animal Health Supervision,Dunhua 133700,China;College of Animal Science,Jilin University,Changchun 130062,China)
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2018年第6期734-739,共6页
Journal of Jilin Agricultural University
基金
白城师范学院博士科研启动基金项目
国家自然科学基金项目(31672514
31772699)
吉林省科技发展计划项目(20170101024JC)
吉林省教育厅项目(JJKH20190017KJ)
