摘要
In this paper,the character of viral clones from early and late passages after serial passages of Trichoplusia in single nuclear polyhedrosis virus in a Tn SB1-4 cell line is described.It demonstrated that no significant difference was observed in the infectivity of the cell culture supernatants of various passages to the cell line.The number of polyhedra produced in a cell and infechvity of polyhedra to T.ni larvae declined strikingly with the increase of passages.The polyhedra without vinons begun to increase from passage to passage.The result of restriction enzyme digestion showed that the DNA restriction fragments of the clones were different from wild virus DNA,although they came from a homogeneous viral DNA.The mutation of viral DNA resulted in the increase of noninfectious polyhedra without vinous and in the increase of the number of polyhedra produced in cell line as wen as virulence of the polyhydrosis inclusion bodys to T.ni larvae after prolonged passages of Tn SNPV in the cell culture.
In this paper,the character of viral clones from early and late passages after serial passages of Trichoplusia in single nuclear polyhedrosis virus in a Tn SB1-4 cell line is described.It demonstrated that no significant difference was observed in the infectivity of the cell culture supernatants of various passages to the cell line.The number of polyhedra produced in a cell and infechvity of polyhedra to T.ni larvae declined strikingly with the increase of passages.The polyhedra without vinons begun to increase from passage to passage.The result of restriction enzyme digestion showed that the DNA restriction fragments of the clones were different from wild virus DNA,although they came from a homogeneous viral DNA.The mutation of viral DNA resulted in the increase of noninfectious polyhedra without vinous and in the increase of the number of polyhedra produced in cell line as wen as virulence of the polyhydrosis inclusion bodys to T.ni larvae after prolonged passages of Tn SNPV in the cell culture.
