摘要
将GFPcDNA片段克隆到昆虫杆状病毒的转移质粒pBacPAK8上,构建成携带绿色荧光蛋白的表达载体pBacPAK8 GFP;采用脂质体共转染法,将载体pBacPAK8 GFPDNA和亲本病毒Ac BacPAK6DNA共转染Sf21细胞,荧光均匀地分布于整个细胞。病毒经空斑分离法,成功分离病毒克隆,获得了重组绿色荧光蛋白的杆状病毒。
The gfp DNA fragments were amplified and inserted into baculovirus expression vector pBacPAK8. pBacPAK8-GFP DNA and viral Ac-BacPAK6 DNA were cotransfected into Sf21 cells with lipofectin. Observation under fluorescence microscope showed that the green fluorescence was spread in entire Sf21 cells,testifying expression of gfp to be easily detectable. The baculovirus plaques were picked up and the clones of baculovirus were isolated successfully.
出处
《安徽农业大学学报》
CAS
CSCD
北大核心
2005年第2期240-242,共3页
Journal of Anhui Agricultural University
基金
江苏省教育厅基金项目(02KJD180003)
江苏大学青年基金资助。
