摘要
利用制备高效液相色谱法从银杏叶中分离制备了聚戊烯醇同系物单体。在HiQsilC18柱上,以异丙醇 甲醇 正己烷 水(体积比为50∶25∶15∶4)为流动相,流速10mL/min,采用等度洗脱方式,制备了8种化合物,经紫外光谱、红外光谱及质谱分析,确认它们分别为C70,C75,C80,C85,C90,C95,C100和C105聚戊烯醇,其中主要成分为C85及C90聚戊烯醇。高效液相色谱分析表明,制备的C75～C105聚戊烯醇化合物的纯度均在96%(质量分数)以上。在该色谱条件下,各色谱峰达到了基线分离,且分离时间短,溶剂用量少。
A method using preparative high performance liquid chromatography (HPLC) to isolate individual polyprenols from a mixture of polyprenols in the leaves of Ginkgo biloba L. is reported. A preparative HiQ sil C18 column was employed in the procedure. By isocratic elution with isopropyl alcoholmethanolhexanewater (50∶25∶15∶4,v/v) as the mobile phase at a flow rate of 10 mL/min, eight polyprenols were obtained from the leaves, and subsequently were identified by ultraviolet, infrared and mass spectroscopic methods as prenol70, 75, 80, 85, 90, 95, 100 and 105. Prenol85 and prenol90 were found to be the main components. HPLC analytical results showed that the purity of each polyprenol (prenol75- 105) was over 96%. Under the chromatographic conditions, every peak was completely baseline separated and consumption of solvents was low. The method was simple and may be employed to provide enough amounts of individual polyprenols for bioactivity, biochemical and biophysical study of polyprenols in the leaves of Ginkgo biloba L.
出处
《色谱》
CAS
CSCD
北大核心
2003年第1期49-51,共3页
Chinese Journal of Chromatography
基金
国家自然科学基金资助项目(批准号:29976018).
关键词
高效液相色谱法
聚戊烯醇
分离
制备
银杏叶
high performance liquid chromatography
polyprenol
separation
preparation
leaf ofGinkgo biloba L.
