摘要
目的 :为进一步了解 2型糖尿病患者血小板活化情况。方法 :采用流式细胞术检测了 4 5例 2型糖尿病患者及 2 6例正常对照者血小板表面血小板膜糖蛋白GPⅠb(CD4 2b)、GPⅡb(CD4 1a)、P -选择素 (CD6 2 p)及血小板激活复合物 (PAC - 1)的表达。结果 :糖尿病患者血小板膜糖蛋白P -选择素 (P =0 .0 4 )及PAC - 1(P =0 .0 0 )表达的荧光强度较正常对照组明显升高 ;而GPⅠb表达的荧光强度较正常对照组显著下降 (P =0 .0 1) ;GPⅡb表达则无明显改变 (P =0 .2 9)。结论 :糖尿病患者血小板活化明显增强 ,PAC - 1的表达可作为GPⅡb/Ⅲa激活更敏感的新型特异性标志。
Objective:To further study the activation of platelet in type 2 diabetes mellitus.Methods:By flow cytometry analysis, we examined platelet membrane glycoprotein GPⅠb (CD42b) , GPⅡb (CD41a), P-selectin(CD62p) and Platelet activation complex (PAC-1) in 45 patients with type 2 diabetes mellitus and 26 normal control people. Results:In diabetic patients,the fluorescence intensity of P-selectin ( P =0.04) and PAC-1 expression ( P =0.00) were elevated in contrast to normal obviously. The GPⅠb fluorescence intensity in diabetic patients was markedly decreased( P =0.01), but GPⅡb fluorescence intensity had no change( P =0.29). Conclusions:Platelet activation in diabetes is elevated obviously. The PAC-1 expression might be a more sensitive and specific marker of activated GPⅡb/Ⅲa.
出处
《中国现代医学杂志》
CAS
CSCD
2003年第2期7-9,共3页
China Journal of Modern Medicine
基金
温州医学院资助课题 (基金编号 2 0 0 0 2 1)
