摘要
目的:探讨中成药熄风胶囊对戊四唑致痫小鼠脑一氧化氮合酶(NOS)蛋白表达的影响。方法:50只昆明种小鼠,按随机方法分为5组,即熄风胶囊3个剂量组、模型组、正常对照组。将不同浓度的熄风胶囊浓缩剂分别灌服三组小鼠,连续6d。前4组腹腔注射戊四唑(55ml/kg)致痫,55min后处死动物。采用免疫组织化学法观察熄风胶囊对戊四唑致痫小鼠脑神经元型一氧化氮合酶(nNOS)蛋白表达的影响。结果:熄风胶囊各组nNOS阳性细胞数目明显多于模型组(P<0.01)。熄风胶囊3个剂量组在海马、皮质层、杏仁核各组均有差异(P<0.05)。结论:熄风胶囊对癫痫小鼠海马神经元有保护作用,此结果可能是其治疗癫痫的机理之一。
Objective: To study the effect of Chinese patent medicine Xifeng capsule on the expression of protein in hippocampul subareas of epileptogenic rat by pentylenetetrazol(PTZ). Methods: Fifty rats were randomly divided into five groups, including three experimental groups,model group,normal control group. The Chinese patent medicine Xifeng capsule was made into three different concentrated solution. The three experimental group of rats was fed that solution individually six days. Then four group rats among them was intraperitoneal inject PTZ, the animal were sacrificed on the 55 minutes and the brains were removed. Immunohistochemical method were used to show the change of neuron type nitric oxide synthase( nNOS) in different hippocampal subareas of epileptogenic rat. Results: The number of nNOS positive neurons in experimental group rats is larger than that of modal group in CA1、CA3 and dentate gyrus areas. Still the difference in CA1 area was found among three experimental group. Conclusion: Xifeng capsule has protective function to those nerve cells. The results may be one of the mechanisms of that medicine to cure epileptic.
出处
《中国中医药信息杂志》
CAS
CSCD
2003年第1期14-15,共2页
Chinese Journal of Information on Traditional Chinese Medicine
基金
天津市教委课题(№.98C034)
关键词
熄风胶囊
癫痫
一氧化氮合酶
蛋白表达
中医药疗法
Xifeng capsule
epileptic
neuron type nitric oxide synthase(nNOS)
expression of protein
