摘要
目的:观察复方石菖蒲颗粒(CSCP)对戊四唑(PTZ)致痫小鼠行为及海马结构的影响。方法:昆明种小鼠60只,雌雄各半随机分为6组:空白组,模型组,苯妥英钠组,CSCP高(4 000 mg·kg-1)、中(2 000 mg·kg-1)、低(1 000 mg·kg-1)剂量组,空白组和模型组给予等体积生理盐水除外,其他组别给予对应药物,连续灌胃7天,末次给药1 h后,按80 mg·kg-1剂量腹腔注射PTZ(空白组除外),造成急性癫痫模型,观察CSCP对PTZ致痫小鼠的发作潜伏期、行为分级、发作次数等的影响;采用脑组织切片HE染色观察海马结构的改变。结果:CSCP可以改善癫痫小鼠的发作程度,延长轻度发作潜伏期,减少Ⅳ、Ⅴ级癫痫发作次数;观察HE染色切片可见PTZ致痫小鼠海马结构发生改变,细胞数量减少,苯妥英钠和CSCP可有效保护小鼠海马神经元。结论:CSCP对PTZ致痫小鼠有一定抑制作用及减少海马神经元细胞的损伤,其抗癫痫机制有待进一步探讨。
This study was aimed to observe the effect of Compound Shi-Chang-Pu （CSCP） granula on behavior, structure changes of hippocampus with epileptic seizure of mice induced by pentyenetetrazole （PTZ）. Sixty Kunming mice, which were half male and half female, were randomly divided into 6 groups, which were the blank control group, animal model, phenytoin （PHT） group, high-dose CSCP （4 000 mg·kg^-1） group, middle-dose CSCP （2 000 mg·kg^-1） group and low-dose CSCP （1 000 mg·kg^-1） groups. The blank group and model group were given intragas-tric administration the same volume of saline, the others animals treated with continuous oral administration of drugs for 7 days. Intraperitoneal injection of PTZ （80 mg·kg^-1） was given 1 h after the last medication to establish the a-cute epilepsy model （expect the blank control group）. Observation was made on the influence of CSCP on incubation period, behavior degree and times of epileptic seizure among PTZ induced epilepsy mice, the structure changes of mice were detected by the brain tissue HE staining. The results showed that CSCP can improve the degree of seizure attack, prolong the incubation period of mild attack, and decrease Ⅳ and Ⅴ degree epileptic seizure, the PTZ in-duced epilepsy mice could damage the structure of hippocampus, reduce the number of cells, PHT and CSCP could ameliorate these changes. It was concluded that CSCP had certain inhibition on epileptic seizure mice induce by PTZ and ameliorate the damage of hippocampus. The antiepileptic mechanism still requires futher study.
出处
《世界科学技术-中医药现代化》
北大核心
2014年第5期1014-1019,共6页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
广西科技厅科技基础条件平台建设(12-97-20):广西麻鸭病毒性肝病动物模型的建立
负责人:黄仁彬
广西科技厅自然科学基金重点项目(2011GXNSFD018030):玉郎伞多糖胶囊抗老年性痴呆的成药性研究
负责人:焦杨
南宁市科技局科技攻关与新产品试制(201102084C):玉郎伞多糖胶囊抗老年性痴呆的临床前研究
负责人:黄仁彬
关键词
癫痫
复方石菖蒲
行为学
戊四唑
海马
Epilepsy
Compound Shi-Chang-Pu granules
behavior
pentylenetetrazol
hippocampus
