摘要
SCID小鼠是一种T和B淋巴细胞严重缺陷的动物,是肿瘤学和免疫学等学科研究的很有用的模型。 将SCID小鼠饲养于塑料薄膜隔离器内,保持无特定病原体状态。其平均每窝产仔数为3.4只,不育率为27.2％,离乳率为73.5％。该小鼠胸腺无皮质结构,髓质保留,缺乏淋巴细胞;脾脏内滤泡呈淋巴细胞脱空状态;淋巴结皮质区不明显,整个淋巴结呈淋巴细胞脱空状态;小肠粘膜下淋巴样组织无淋巴细胞聚集。外周血淋巴细胞仅占白细胞总数的10-24％。该小鼠基本符合免疫学试验要求。
Mice with severe combined immunodeficiency (SCID) disease are deficient for both T and B lymphocyte functions because of impairment of lymphoiesis. To ensure SCID mice to survive, we raised them in a plastic film isolator in which specific-pathogen-free (SPF) condition was kept, free of 12 viruses and 17 bacteria considered as common pathogens of mice, as well as endoparasites and ectoparasites.Body weights of the SCID mice 4 and 8 weeks old were the same as those of the control group of BALB/c mice. Average litter size of the SCID mice was 3.4, slightly smaller than that of BALB/c mice, but their non-productive rate and weaned-to-born ratio were comparable to those of BALB/c mice, i. e. 27.2% and 73.5%, respectively.Circulating lymphocytes of SCID mice only comprised a small fraction of the total leukocytes of peripheral blood (10-24%), while neutrophils were 76-88%. Conversely, in BALB/c mice, lymphocytes were 75-87% and neutrophils 15-25%. Theymus glands of the SCID mice were abnormally small, and their relative weight was only about 6% of that of BALB/c mice. SCID thymus was found to consist of a rudimentary medulla without cortex and contained few lymphocytes, predominated by epithelioid cells and fibroblasts. Relative weight of SCID spleen was about 30% of that of BALB/c mice. SCID spleen follicles were empty, virtually devoid of lymphocytes, occupied by reticular cells. But red pulp of SCID spleens was normal as in BALB/c mice. SCID lymph nodes were extremely small, only 1/3-1/4 of normal size of BALB/c mice. SCID lymph nodes showed no clear cortex structure, devoid of paracortical area, lacking follicles. The whole structure of SCID lymph nodes was poorly populated by lymphocytes, but predominated by reticular cells. SCID lymphoid tissues in submucosa of small intestines were undeveloped. The follicular-like sites were mainly composed of reticular cells without lymphocytes.The SCID mice may be sued to study lymphoid differentiation, and serve as an in vivo test system for transplanted tumors and normal ti
出处
《动物学报》
SCIE
CAS
CSCD
1992年第3期309-317,共9页
ACTA ZOOLOGICA SINICA
基金
国家自然科学基金
关键词
SCID
小鼠
免疫组织
饲养
繁殖
SCID mice, Husbandry, Reproduction, Immune tissues.
