摘要
目的:探讨超抗原葡萄球菌肠毒素B(SEB)诱导的耐受性是否参与免疫赦免部位的应答反应。方法:以LEW大鼠作为受体,F344大鼠作为供体,进行角膜移植。将受体鼠随机分成5组,即在手术前7 d及移植后7 d,以球旁途径分别注射30μg/kg、60μg/kg、90μg/kg和120μg/kg的SEB组和注射生理盐水的阴性对照组。阳性对照组分别为移植后注射糖皮质激素(GC)、FK506、环孢素A((CsA)和白介素-1受体拮抗剂(IL-1 ra)组。镜下观察移植后30 d内角膜的浑浊、水肿和新生血管,用抗排斥反应指数记录移植片存活天数。采用NK1.1-PE荧光抗体进行组织染色,观察移植后不同时间受体鼠角膜片的炎症细胞浸润和受体鼠眼局部NK细胞的变化。结果:注射120μg/kg SEB的大鼠移植片比生理盐水对照组的存活天数延长了22 d。与FK506、CsA、IL-1ra和GC组相比也明显延长。120μg SEB/kg受体鼠的移植片排斥反应指数为3.42±2.18,明显低于对照组6.58±3.15(P<0.01)。其中水肿指数和新生血管指数明显降低。组织染色结果显示,SEB注射后NK细胞数量增加。结论:注射SEB能降低大鼠角膜移植的排斥反应,提示SEB诱导的耐受性参与了免疫赦免部位的应答反应。
AIM: To investigate if the immunological tolerance induced by superantigen staphylococcus enterotoxin B (SEB) takes part in the reaction at the immune privileged site. METHODS: Corneal transplantations were carried out using LEW rats as recipient and F344 rats as the donor. The recipient rats were randomly divided into 5 groups, receiving peribulbar SEB injection of a dosage of 30, 60, 90 or 120 ug/kg 7 days before and after operations. The negative control group received saline injection while the positive control group received glu-cocorticoid(GC), FK506, CsA or interleukin-1 receptor an-tagon ist (IL-1ra), respectively. The survival time and rejection index of corneal grafts within 30 days postoperation were examined and recorded using anti-rejection index method. The inflammatory cell infiltration of the recipient cornea and changes of local NK cells were examined by PE-conjugated anti-NK1. 1 monoclonal antibody. RESULTS: The grafts in the group receiving injection of 120 ug/kg SEB survived 22 days longer than the negative control group. Their survival time is also significantly longer than the groups treated with FK506, CsA, GC or IL-lra. The graft rejection index was 3.42 ±2.18 in the rats receiving 120 ug SEB/kg, but 6. 58 ± 3. 15 in the negative control group (P <0. 01). The edema and new blood vessel indexes were also significantly reduced. The immunostaining showed that the number of NK cells increased following injection of SEB. CONCLUSION: Injection of SEB reduced the rejection to corneal transplants in rats, showing that the immune tolerance induced by superantigen SEB plays a role in the reaction at the immune privileged site.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2003年第1期26-28,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助(No.30271385)
军队总后卫生部基金资助(2000卫中技字-71)
