摘要
目的探讨lncRNA HIF1A-AS1在结直肠癌患者血清中的表达及其与临床病理特征及预后的关系。方法结直肠癌患者105例为结直肠癌组,体检健康者100例为对照组,采用实时荧光定量PCR检测2组血清lncRNAHIF1A-AS1相对表达量。将结直肠癌组以血清lncRNA HIF1A-AS1相对表达量中位数分为高表达组(lncRNAHIF1A-AS1相对表达量≥1.51)和低表达组(lncRNA HIF1A-AS1相对表达量<1.51),分析血清lncRNA HIF1A-AS1相对表达量与结直肠癌临床病理特征间关系;绘制ROC曲线,分析血清lncRNA HIF1A-AS1表达水平对结直肠癌的诊断效能;采用Kaplan-Meier法绘制生存曲线,评估血清lncRNA HIF1A-AS1表达对结直肠癌患者生存率的影响;采用Cox比例风险回归模型分析结直肠癌预后不良的危险因素。结果结直肠癌组血清lncRNA HIF1A-AS1相对表达量(2.81±0.02)高于对照组(0.97±0.01)(P<0.05);以血清lncRNA HIF1A-AS1相对表达量1.82为最佳截断值,lncRNA HIF1A-AS1诊断结直肠癌的AUC为0.873(95%CI:0.830～0.916,P<0.001),灵敏度为75.5%,特异度为96.0%;高表达组肿瘤低分化(25.00%)、肿瘤直径>5cm(48.08%)、T_3～T_4期(46.15%)、N_(1-3)期(40.38%)、M_1期(50.00%)、TNM分期Ⅲ～Ⅳ期(67.31%)比率明显高于低表达组(20.75%、24.53%、22.64%、26.42%、37.74%、37.74%)(P<0.05),总生存时间[(481±37)d]短于低表达组[(695±53)d](P<0.05),年龄、性别比例和肿瘤位置与低表达组比较差异无统计学意义(P>0.05);血清lncRNA HIF1A-AS1高表达是结直肠癌患者预后不良的独立危险因素(HR=3.165,95%CI:1.218～5.085,P=0.008)。结论血清lncRNA HIF1A-AS1在结直肠癌患者中表达上调,其可作为结直肠癌早期诊断和评估预后的潜在生物标志物。
Objective To investigate the expression of serum lncRNA HIF1 A-AS1 in colorectal carcinoma(CRC)and its correlation with the clinicopathological features and prognosis of CRC.Methods Real-time fluorescence PCR was used to measure the relative expressions of serum lncRNA HIF1 A-AS1 in 105 patients with CRC(CRC group)and 100 healthy volunteers(control group).CRC group was redivided into high expression group(lncRNA HIF1 A-AS1 relative expression≥1.51)and low expression group(lncRNA HIF1 A-AS1 relative expression<1.51)according to the median of relative expression of lncRNA HIF1 A-AS1.The relationship between the relative expression of serum lncRNA HIF1 A-AS1 and clinicopathological features of CRC was analyzed.ROC was used to evaluate the diagnostic efficiency of serum lncRNA HIF1 A-AS1 on CRC.Kaplan-Meier analysis was performed to evaluate the influence of lncRNA HIF1 A-AS1 on the survival rate of CRC patients.Cox proportional hazards regression model was used to analyze the risk factors for poor prognosis of CRC.Results The relative expression of lncRNA HIF1 A-AS1 was significantly higher in CRC group(2.81±0.02)than that in control group(0.97±0.01)(P<0.05).When the optimal cut-off value of lncRNA HIF1 A-AS1 was 1.82,the AUCvalue for CRC was 0.873(95%CI:0.830-0.916,P<0.001),the sensitivity was 75.5% and specificity was 96.0%,respectively.The proportations of poorly differentiated tumor(25.00%),tumor diameter>5 cm(48.08%),T3-T4 stage(46.15%),N1-3 stage(40.38%),M1 stage(50.00%)and TNM stageⅢ-Ⅳ(67.31%)in high expression group were significantly higher than those in low expression group(20.75%,24.53%,22.64%,26.42%,37.74%,37.74%)(P<0.05).The overall survival time was significantly shorter in high expression group((481±37)d)than that in low expression group((695±53)d)(P<0.05).There were no significant differences in age,gender and tumor location between high and low expression groups(P>0.05).High expression of serum lncRNA HIF1 A-AS1 was an independent risk factor for poor prognosis of CRC(HR=3.165,95%CI:1.218-
作者
郑凤萍
罗巨利
王爱华
ZHENG Fengping;LUO Juli;WANG Aihua(Department of Anorectal Surgery,the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha410007,China)
出处
《中华实用诊断与治疗杂志》
2019年第2期143-146,共4页
Journal of Chinese Practical Diagnosis and Therapy
基金
湖南省科技计划项目(S2012F1023)
湖南省科技厅项目(W2012ZT71)
