摘要
目的研究探讨肝癌组织中复制蛋白A1(RPA1)的表达变化及其临床意义。方法选择2015年1月至2018年5月就诊的82例肝癌患者为研究对象,采集肝癌患者的肝癌组织、癌旁正常组织,采用逆转录聚合酶链反应(RT-PCR)、蛋白质印迹法(Western blot)对肝癌组织、癌旁组织中的RPA1 mRNA及蛋白表达水平进行检测。比较肝癌组织与癌旁组织的RPA1 mRNA、RPA1蛋白表达水平。比较乙肝表面抗原阳性与阴性患者、淋巴结转移与无转移患者、不同肿瘤TNM分期患者、不同病理分化程度患者肝癌组织的RPA1 mRNA、RPA1蛋白表达水平,并分析RPA1 mRNA、RPA1蛋白表达水平与肝癌患者临床病理特征的相关性。结果 (1)肝癌与RPA1:肝癌患者肝癌组织的RPA1 mRNA、RPA1蛋白表达水平均高于癌旁组织(P <0. 01)。(2)肝癌临床病理特征与RPA1:淋巴结转移患者的RPA1 mRNA、RPA1蛋白表达水平均高于无淋巴结转移患者(P <0. 01); TNMⅢ～Ⅳ期患者的RPA1 mR-NA、RPA1蛋白表达水平均高于TNMⅠ～Ⅱ期患者(P <0. 01);肿瘤低分化患者的RPA1 mRNA、RPA1蛋白表达水平均高于肿瘤中高分化患者(P <0. 01)。相关性分析发现,RPA1 mRNA、RPA1蛋白表达水平与肝癌患者的淋巴结转移、肿瘤分期呈正相关,与病理分化程度呈负相关(P <0. 05),而与乙肝表面抗原阳性之间无相关性(P>0. 05)。结论 RPA1 mRNA、RPA1蛋白表达水平在肝癌组织中普遍处于增高状态,其表达水平与淋巴结转移、肿瘤分期、病理分化程度等临床病理特征存在紧密联系,临床上可将PRA1作为肝癌早期诊断、疗效评估及预后预测的辅助标志物。
Objective To investigate the expression of replication protein A1( RPA1) in hepatocellular carcinoma( HCC)tissue and its clinical significance. Methods Eighty-two patients with hepatocellular carcinoma from January 2015 to May2018 were selected as study subjects,in whom liver cancer tissues and adjacent normal tissues were collected. Reverse transcription polymerase chain reaction( RT-PCR) and Western blot were used to detect RPA1 mRNA and protein expressions in hepatocellular carcinoma and para-cancerous tissues. The expression levels of RPA1 mRNA and RPA1 protein in hepatocellular carcinoma and adjacent tissues were compared. The expression levels of RPA1 mRNA and RPA1 protein in hepatocellular carcinoma tissues were compared in the patients with positive or negative hepatitis B surface antigen( HBs Ag),with lymph node metastasis and non-metastasis,with different TNM stages and with different pathological differentiation. The correlation between the expressions of RPA1 mRNA and RPA1 protein and the clinicopathological features of HCC was analyzed. Results( 1) The expression levels of RPA1 mRNA and RPA1 protein in HCC tissues were significantly higher than those in adjacent tissues( P < 0. 05).( 2) The expression levels of RPA1 mRNA and RPA1 protein in patients with lymph node metastasis and poorly differentiation and at TNM Ⅲ-Ⅳ stage were respectively higher than those in patients without lymph node metastasis,with high differentiation and at TNM stage Ⅰ-Ⅱ( P < 0. 05). The correlation analysis showed that the expression levels of RPA1 mRNA and RPA1 protein were positively correlated with lymph node metastasis and tumor stage and negatively correlated with the degree of pathological differentiation( P < 0. 05),and there was no linear correlation between them and positive HBs Ag( P > 0. 05). Conclusions RPA1 mRNA and RPA1 protein expression levels generally increase in HCC tissues and are closely related to lymph node metastasis,tumor stage,pathological differentiation and so on. PRA1 can be used as an au
作者
王肖泽
田学军
蒙轩
WANG Xiao-ze;TIAN Xue-jun;MENG Xuan(Department of General Surgery,Yuquan Hospital of Tsinghua University,Beijing 100040,China)
出处
《中国临床研究》
CAS
2019年第2期229-231,共3页
Chinese Journal of Clinical Research
基金
国家自然科学基金(81502376)~~
关键词
复制蛋白A1
肝癌
诊断
淋巴结转移
肿瘤分期
分化程度
Replication protein A1
Hepatocellular carcinoma
Diagnosis
Lymph node metastasis
Tumor stage
Differentiation degree
