摘要
目的:通过检测支气管灌洗(BAL)液与血清中趋化因子(IL-8),分泌性白细胞蛋白酶抑制剂(SLPI),可溶性细胞间粘附分子-1(s ICAM-1)和s CD14含量,探讨IL-8、SLPI,s CD14和s ICAM-1作为具有相似病程的哮喘和COPD患者炎症和免疫应答反应生物标志物的潜力。方法:采用酶联免疫法(ELISA)测定哮喘(n=13)与COPD(n=25)患者血清和支气管灌洗液中IL-8,SLPI,s CD14和s ICAM-1含量。结果:哮喘和COPD患者BAL中IL-8和SLPI含量远高于血清中IL-8和SLPI含量,而BAL中s ICAM-1和s CD14含量远低于血清中s ICAM-1和s CD14含量。在测定的所有生物标志物中,COPD患者中只有BAL IL-8的浓度高于哮喘患者BAL IL-8的浓度。两组中,BAL IL-8与SLPI相关。COPD患者中,BAL s ICAM-1和s CD14相关,而哮喘患者中BAL s ICAM-1与FEV1/FVC相关。此外,哮喘患者中血清中SLPI与s CD14相关,且血清中s ICAM-1与FEV1/FVC负相关。结论:分析特定生物标志物时,选择正确的生物流体至关重要。在测定的4个生物标志物中,COPD患者中只有BAL IL-8浓度高于哮喘患者BAL IL-8浓度。
Objectvie To quantify bronchial lavage(BAL) fluid and serum levels of chemokine(IL-8), secretory leukocyte protease inhibitor(SLPI), soluble intracellular adhesion molecules-1(s ICAM-1) and s CD14, as surrogate markers of inflammatory and immune response in asthma and chronic obstructive pulmonary disease(COPD) patients with similar disease duration time. Methods Biomarkers in serum and BAL fluid from asthma(n =13) and COPD(n=25) patients were detected by ELISA. Results We found that in asthma and COPD groups the concentrations of IL-8 and SLPI are significantly higher in BAL fluid than in serum, while levels of s ICAM-1 and s CD14 in BAL fluid are significantly lower than in serum. Of these 4 measured biomarkers, only the BAL IL-8 was higher in COPD patients when compared to asthma. In both groups, BAL IL-8 correlated with SLPI. In patients with COPD the BAL s ICAM-1 correlated with s CD14, while in asthma patients BAL s ICAM-1 correlated with FEV1/FVC. Moreover, in asthma patients the serum SLPI correlated with s CD14 and serum s ICAM-1 negatively correlatedwith FEV1/FVC. Conclusion Our findings point to the importance of selecting a correct biological fluid when analyzing specific biomarkers, and also show that of 4 measured biomarkers, only the BAL IL-8 was higher in COPD patients when compared to asthma.
出处
《湖南师范大学学报（医学版）》
2016年第3期116-119,120,共5页
Journal of Hunan Normal University(Medical Sciences)
