摘要
植物C_2H_2型锌指蛋白是植物转录调节因子中的大家族,通过转录调控、翻译后调节和蛋白质间的相互作用在植物发育和环境胁迫中发挥关键作用.该研究在水稻基因组中克隆到一个C_2H_2型锌指蛋白,命名为OsZAT12.生物信息学分析显示OsZAT12基因全长597 bp,其中不包含内含子,其蛋白质序列含有199个氨基酸,具有2个典型的C_2H_2锌指结构. OsZAT12蛋白定位于细胞核,在根中特异性表达.过表达拟南芥植株与野生型比较,其根变短,子叶的变绿率明显降低,植株矮小.初步推断OsZAT12基因可能影响植物生长发育,尤其是根的伸长.
The C2H2-type zinc finger proteins are a large family among the factors that regulate plant transcription and play a key role in plant development and environmental stress through transcriptional regulation,post-translational regulation,and protein-protein interactions. In this study,a C2H2-type zinc finger protein in the rice genome was cloned and named as OsZAT12. The bioinformatics analysis showed that gene OsZAT12 is 597 bp in length and does not include introns,and its protein sequence contains 199 amino acids with two typical C2H2 zinc finger structures. OsZAT12 is localized in the nucleus and specifically expressed in roots. Compared with its wild counterpart,Arabidopsis in which OsZAT12 is overexpressed has shorter roots,a significantly lower greening rate of cotyledons,and a smaller size. A preliminary conclusion is that OsZAT12 may affect the growth and development of plant,especially root elongation.
作者
陈宗新
陈俊飞
王亚琴
CHEN Zongxin;CHEN Junfei;WANG Yaqin(School of Life Sciences,Guangdong Provincial Key Lab of Biotechnology for Plant Development,South China Normal University,Guangzhou 510631,China)
出处
《华南师范大学学报（自然科学版）》
CAS
北大核心
2019年第1期63-68,共6页
Journal of South China Normal University(Natural Science Edition)
基金
国家自然科学基金项目(31672188)
广东省应用型科技研究专项项目(2015B020231009)
