摘要
目的探讨乳腺癌中miR-155的表达与临床病理特征的关系及与人表皮生长因子受体3(human epidermal grouth factor receptor-3,HER-3)蛋白的相关性。方法选取手术切除的乳腺癌标本和癌旁组织标本选择同期30例乳腺部良性病变患者穿刺活检获得的组织标本,采用实时荧光聚合酶链式反应(quantitative real-time-polymerase chain reaction,qPCR)检测miR-155的表达,以及采用免疫组织化学染色检测HER-3蛋白的表达。结果 miR-155在乳腺癌组织中的表达水平均明显高于癌旁组织与良性病变组织(P<0.05)。乳腺癌组织中miR-155的表达与TNM分期、组织分级、淋巴结转移有关(P<0.05)。HER-3在乳腺癌组织中的表达水平均明显高于癌旁组织与正常组织(P<0.05)。miR-155与HER-3蛋白表达之间呈正相关(r=0.428,P<0.05)。结论乳腺癌生物学行为和HER-3蛋白的表达共同促进了miR-155的过度表达。
Objective To explore the relationship between expression of miR-155 and clinicopathological features,and its correlation with human epidermal growth factor receptor-3(HER-3) protein in breast cancer. Methods The surgical specimens of breast cancer and paracancer tissue specimens were selected, and the normal tissue specimens obtained by puncture biopsy of benign breast lesions in the same period were collected.The expression of miR-155 was detected by quantitative real-time-polymerase chain reaction(qPCR), the expression of HER-3 protein was detected by immunohistochemical staining. Results The expression level of miR-155 in breast cancer tissues was significantly higher than that in paracancer tissues and normal tissues(P<0.05).The expression of miR-155 in breast cancer tissues was correlated with TNM stage, tissue grading and lymph node metastasis(P<0.05). The expression level of HER-3 protein in breast cancer tissues was significantly higher than that in paracancer tissues and normal tissues(P<0.05). The expression level of miR-155 was positively correlated with the expression of HER-3 protein(r=0.428, P<0.05).Conclusion The overexpression of miR-155 is closely related to the biological behavior of breast cancer and positively correlated with the expression of HER-3 protein.
作者
罗思佳
张建新
张智勇
金炜东
LUO Sijia;ZHANG Jianxin;ZHANG Zhiyong;JIN Weidong(Department of General Surgery,General Hospital of Central Theater Command,Wuhan Hubei 430070,China)
出处
《华南国防医学杂志》
CAS
2019年第5期312-315,共4页
Military Medical Journal of South China
