摘要
为了克隆出水貂EDNRB基因的启动子区域,预测该片段的核心启动子区域、CPG岛、顺式作用元件及转录因子结合位点,为水貂EDNRB基因的表达调控研究提供理论依据,试验采用PCR扩增、克隆及生物信息学方法进行了研究。结果表明:克隆出的长为1 830 bp的水貂EDNRB基因候选启动子序列,经检验与人的EDNRB基因序列有高度的同源性;在此段序列中利用在线软件成功预测出多个核心启动子区域,2个CPG岛区域,3个GAAT框,1个GC框,6个TATA框,并提示存在MEF2、NF-1、Sp-1等转录因子结合位点。说明水貂EDNRB基因的-1 763/+67 bp候选启动子区域可能受到甲基化影响,同时也受顺式作用元件与转录因子的调控作用。
In order to clone the promoter region of the mink EDNRB gene,the core promoter region,CPG island,cis-acting elements,and transcription factor binding site of the fragment were predicted,providing theoretical basis for the study of the expression and regulation of the mink EDNRB gene.PCR amplification,cloning and bioinformatics methods were used in this study.The results showed that the EDNRB gene candidate promoter sequence of the mink with a length of 1 830 bp had a high homology with human EDNRB gene sequence.In this sequence,online software was used to successfully predict multiple core promoter regions,2 CpG island regions,3 GAAT boxes,1 GC box,6 TATA boxes,and MEF2,NF-1,Sp-1 and other transcription factors binding sites were existed.It indicates that the-1 763/+67 bp candidate promoter region of mink EDNRB gene may be affected by methylation,and it is also regulated by cis-acting elements and transcription factors.
作者
王亚琪
胡露露
王瑞宁
刘铮铸
巩元芳
李祥龙
彭永东
WANG Yaqi;HU Lulu;WANG Ruining;LIU Zhengzhu;GONG Yuanfang;LI Xianglong;PENG Yongdong(College of Animal Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao 066004,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2019年第8期142-145,172,共5页
Heilongjiang Animal Science And veterinary Medicine
基金
国家自然科学基金项目(31501940)
河北省高校创新团队领军人才培育计划项目(LJRC004)
