摘要
采用RT-PCR方法对银黑狐内皮素受体B基困(EDNRB)进行了克隆,序列分析表明所克隆的cDNA序列全长为1 636bp,包含整个开放阅读框1 332bp,编码443个氨基酸残基。银黑狐EDNRB基因编码序列与GenBank数据库中的犬、猪、人、牛、兔和小鼠的EDNRB基因核酸序列高度同源,同源性分别为99.0%,90.7%,89.5%,89.1%,86.9%和82.8%。银黑狐EDNRB基因编码氨基酸序列中包含1个信号肽序列和7个跨膜区域,跨膜区氨基酸序列在不同物种中高度保守。EDNRB基因组织表达谱分析表明该基因在银黑狐肺、脑和肝脏组织中表达量较高,而在肌肉、脾和心脏中表达量较低,在胃组织中没检测到该基因的表达。本研究结果为下一步探讨ED-NRB基因核酸序列多态性与银黑狐不同毛色表型的相关性奠定了基础。
The EDNRB complementary deoxyribonucleic acid was molecularly cloned from the normal silver-black fox lung tissue by reverse transeription-polymerase chain reaction. Sequence analyses indicated that the cloned cDNA of EDNRB was 1 636 bp in length which contained the entire open reading frame of 1 332 bp encoding 443 amino acid residues. Alignment analysis indicated that the nucleotide sequence of the coding region showed a high homology with the EDNRB sequences of dog（99.0%）, pig（90. 7~//oo）, human（89.5%）, cattle（89. 1 ~//0）, rabbit （86.9%o） and mouse（82.8%）, respectively. The deduced amino acid resides contained a signal peptide and seven putative trans- membrane domains which showed highly conserved amino-acid resides with that of other species above mentioned. Results of the tissue expression of EDNRB gene in silver-black fox indicated that EDNRB gene expressed in six of seven tissues. It expressed with the higher abundance in brain, liver and lung, but it was a little present in heart, spleen and muscle. No expression in stomach tissue was detected in this experiment. The data provide a valuable foundation for studying on polymorphisms of EDNRB gene and their association with different phenotypes of coat color in the silver-black fox.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2012年第9期1396-1399,共4页
Chinese Journal of Veterinary Science
基金
国家级"大学生创新性实验计划"资助项目(2011A81140)
