摘要
选用大长北母猪所产仔猪 45头 ,2 1日龄断奶。断奶时宰杀 5头公仔猪作为哺乳对照 ,其余仔猪按公母各半随机分为 2组 ,试验组添加 1.2 %谷氨酰胺。断奶后 14d和 2 8d宰杀仔猪 ,以研究谷氨酰胺对早期断奶仔猪胰腺和小肠胰蛋白酶活性的影响。断奶后 14d,日粮中添加谷氨酰胺可极显著提高胰腺胰蛋白酶相对活性和总活性 (P<0 .0 0 1)以及比活 (P<0 .0 1) ,而对十二指肠、空肠和回肠食糜及粘膜中胰蛋白酶活性无显著影响 ;断奶后 2 8d时 ,日粮添加谷氨酰胺对胰腺、十二指肠、空肠和回肠食糜粘膜中胰蛋白酶活性均无显著影响。对照组仔猪胰腺、十二指肠和回肠胰蛋白酶活性在断奶后 14d已基本恢复到断奶时水平 ,空肠胰蛋白酶活性显著超过断奶时水平 (P<0 .0 5 ) ;断奶后 2 8d胰腺胰蛋白酶比活和总活性极显著高于断奶后 14d(P<0 .0 1) ,胰腺和回肠胰蛋白酶相对活性显著高于断奶后 14d(P<0 .0 5 )。试验组仔猪胰腺和空肠胰蛋白酶相对活性和比活在断奶后 14d极显著超过断奶时水平 (P<0 .0 1) ,达到了对照组断奶后 2 8d时酶活性水平 ;断奶后 2 8d胰腺胰蛋白酶相对活性、比活和总活性维持在断奶后 14d水平 ,而空肠胰蛋白酶相对活性和比活又降至断奶时水平。结果表明 ,早期断奶仔猪日粮中添加谷氨酰胺有助于缓解由于早期?
The experiment was conducted to investigate the effects of glutamine supplementation in weaned piglets on trypsin activities in pancreas tissue,and in duodenum,jejunum and ileum chyme and mucosa.Forty-five piglets,the offsprings of Large-White-Beijing Hei crossbred sows,weaned at the 21 d of age were used,5 of them were killed at the 21 d of age,and the rest wer e divided randomly into two groups:control treatment(basal diet) and glutamine t reatment(basal diet+1.2% glutamine).Glutamine supplementation increased relativ e activities,specific activities(P<0.01) and total activ ities(P<0.05) of pancreatic trypsin at the 35 d of age,but not significantly at the 49 d of age.G lutamine addition did not affect the trypsin activities in small intestinal chym e and mucosa at the 35 d and 49 d of age.Trypsin activities in jejunum of piglet s without glutamine supplementation at 35 d was significantly greater than those at the 21 d of age(P<0.05);Trypsin activities in pancr eas,duodenum and ileu m had no changed at the 35 d of age,but increased significantly at the 49 d of a ge.Relative activities and specific activities of trypsin in pancreas and jejunu m increased significantly(P<0.01) from 21~35 d by gluta mine supplementation, and pancreatic trypsin activities remained the same level until at the 49 d of a ge.The results suggest that glutamine supplementation may be beneficial for pres erving trypsin activities and accelerating the development of trypsin activities .
出处
《中国兽医学报》
CAS
CSCD
北大核心
2003年第1期59-61,共3页
Chinese Journal of Veterinary Science
