摘要
以牛粪和甘蔗叶为堆肥原料,从实验室自制的强制通风发酵罐堆肥的降温阶段筛选出一株具有高效硝化作用的菌株N7。通过生理生化实验等方法对N7进行初步鉴定,验证并从pH、温度、转速、接种量及初始碳氮源浓度方面探究影响其硝化作用的条件。实验结果表明:菌株N7是一种可以利用亚硝酸盐转化成硝酸盐的细菌,在pH为3,温度为30℃,转速为140 r/min,接种量为1%,初始氮源浓度为0.2%,初始碳源浓度为0.05%时,其将亚硝酸根转化为硝酸根的效率达到最高。
With cow dung compost and sugarcane leaves as raw materials,a strain named N7,with great nitrification performance was screened from the lab homemade forced ventilation cooling stage of fermentation. Using physiological and biochemical experiment methods,such as the preliminary identification of the N7,validate and from pH,temperature,rotational speed,inoculation amount and initial concentration of carbon and nitrogen source,the conditions affecting the nitrification were explored. The experimental results show that the strain N7 can make good use of nitrite,converting it into nitrate. When the pH value is at 3,the temperature of 30 ℃,the rotational speed of 140 r / min,1% inoculated quantity,initial concentration of nitrogen source was 0. 2%,the initial concentration of carbon source was 0. 05%,the efficiency of nitrate to nitrite can reach the maximum.
出处
《环境工程》
CAS
CSCD
北大核心
2015年第S1期916-919,共4页
Environmental Engineering
基金
桂林市科学研究与技术开发计划项目(20130107-2)
关键词
硝化细菌
堆肥
鉴定
nitrobacteria
compost
identification
