摘要
通过轮叶黑藻腐解模拟试验,分析了底泥和砂石2种基质条件下营养物质释放和微生物胞外酶活性变化特征。结果表明,试验结束时底泥组和砂石组的轮叶黑藻质量损失率分别为64. 62%和62. 15%。植物腐解过程释放的营养物质使得水体溶解氧含量迅速降低,TN、TP质量浓度迅速增加。试验结束时(146 d),上覆水体的水质基本恢复初始水平。水体有机质在56 d以色氨基酸类蛋白质为主,之后逐渐以类富里酸和类腐殖酸类物质为主。植物腐解过程与微生物胞外酶活性具有一定的响应关系,跟水体水质及植物本身的特性相关。纤维素酶、蔗糖酶密切相关(r=0. 70,P<0. 05),且受植物TOC和水体TN的影响,过氧化氢酶与水体的DO和EC相关(P<0. 05),碱性磷酸酶及脲酶则分别受TP和NH_3-N的抑制调节(P<0. 05)。
The release of nutrientsand microbial enzyme activities were monitored during the decomposition of Hydrilla verticillatum incubated in tanks with sediments or sands.It can be found that the decomposition rates of sediment and sand substrates were 64.62%and 62.15%,respectively.And during the plant decomposition,abundant nutrients were released into overlying water,and the dissolved oxygen concentrations and oxidationreduction potential values decreased while the concentrations of total nitrogen and total phosphorus rapidly increased.At the end of decomposition(146 d),the quality of overlying water was recovered back to the initial level.The aromatic protein was the main dissolved organic matter into overlying water at first,then the humic acidand fulvic acid-like refractory organic compounds increased gradually.The decomposition process of plant has a relationship with microbial enzyme activities,which is related to the water quality and the characteristics of plant itself.Cellulase and sucrase have a close relationship(r=0.70,P<0.05),which is affected by total organic carbon and total nitrogen.Meanwhile,the catalase has a significant correlation between dissolved oxygen and electrical conductivity in overlying water(P<0.05),while alkaline phosphatase and urease had a negatively correlation with the total phosphorus and the ammonia nitrogen respectively(P<0.05).
作者
张松贺
张丽莎
韩冰
严玲玲
周甜甜
ZHANG Songhe;ZHANG Lisha;HAN Bing;YAN Lingling;ZHOU Tiantian(College of Environment,Hohai University,Nanjing 210098,China)
出处
《河海大学学报（自然科学版）》
CAS
CSCD
北大核心
2019年第2期95-101,共7页
Journal of Hohai University(Natural Sciences)
基金
国家自然科学基金(51579075)
江苏省自然科学基金优秀青年基金(BK20160087)
关键词
轮叶黑藻
植物腐解
底泥
砂石
胞外酶
Hydrilla verticillatum
decomposition of plant
sediment
sand
enzymes
