摘要
目的:探讨已受雌、孕激素影响的人子宫内膜细胞.受一氧化氮作用引起细胞生理变化的机制。方法:取人分泌中期子宫内膜。体外原代培养24—28小时,用钙荧光探剂Fluo-3-AM负荷钙离子,采用激光扫描共聚焦显微镜测定加入一氧化氮合酶抑制剂L-NAME后,子宫内膜细胞内游离钙离子[Ca^(2+)]i浓度的动态变化。结果:加入L-NAME后,腺上皮细胞和间质细胞[Ca^(2+)]i立即开始升高,约在加入后400秒上升到高峰,加入后900秒稍有下降,然后继承升高并保持在高水平。同时发现腺上皮细胞和间质细胞对L-NAME的反应不同,其中腺上皮细胞[Ca^(2+)]i升高的幅度显著高于间质细胞。结论:在雌、孕激素作用的基础上,NO通过改变子宫内膜细胞[Ca^(2+)]i进行信号转导,实现其生理效应。
Objective:To investigate the mechanism of nitric oxide acting to human endometrial cells under the regulation of estrogen and progesterone. Methods: Human endometrial cells were obtained from patients in the implantation window phase of their cycle during endometrium biopsy, and were cultured in vitro. After 24-48 hours of culture, the cells were incubated with Fluo-3AM, and then were stimulated by the NO synthase inhibitor(L-NAME). The dynamic change of intracellular calcium concentration([Ca^(2+)]i)were determined by the laser scanning eonfocal microscopy. Results: [Ca^(2+)]i was up regulated after adding L-NAME immediately. The fluorescent value was highest at 400 seconds, reduced slightly at 900 seconds, then elevatory and retentive in a high level, We observed that the up regulating range in epithelial glandular cells was higher than that of the stromal cells, After the addition of L-NAME, [Ca^(2+)]i was immediately increased, and reached its peak value at 400 second, At 900 second [Ca^(2+)]i was slightly decreased, then risen again and maintained at a high level within the testing period. In adddition, it was observed that the increasing range of [Ca^(2+)]i in epithelial glandular cells was significantly higher than that of the stromal cells. Conclusion: L-NAME could promote the releasing of intracellular calcium from organelle of endometrial cells, witch would probably involve the mechanism of the signal transduction in endometrium.
出处
《福州总医院学报》
2002年第2期93-95,共3页
Journal of Fuzhou General Hospital
