摘要
目的:研究川芎嗪注射液(四甲基吡嗪,TMP)对庆大霉素(GM)耳中毒豚鼠耳蜗螺旋神经节热休克蛋白70(HSP70)表达的影响,探讨TMP对GM耳毒性损伤的保护作用。方法:应用SABC免疫组化技术、原位杂交技术及图像分析技术并结合听性脑干反应(ABR)测试,观察TMP对GM耳中毒豚鼠ABR阈值和耳蜗螺旋神经节HSP70和HSP70mRNA表达的影响。结果:TMP加GM组耳蜗螺旋神经节HSP70及HSP70mRNA的表达和ABR阈值明显低于GM组(P<0.01)。结论:TMP可降低耳蜗螺旋神经节HSP70及HSP70mRNA的表达,以减轻GM的耳毒性损伤,从而改善听功能。
To explore the protective role of ligustrazine (tetramethylpyrazine, TMP) on gentamicin oto-toxicity. Methods: Using immunohistochemistry staining, in situ hybridization histochemistry and image quantitative analysis system, combined with auditory brainstem response (ABR) to observe the effects of TMP on threshold of ABR and the heat shock protein 70 (HSP70) and HSP70mRNA expression in cochlear spiral ganglion of gentamicin intoxicated guinea pigs. Results; The ABR threshold, and HSP70 and HSP70mRNA expression in cochlear spiral ganglion cells of gentamicin intoxicated guinea pig significantly increased, which could be reduced significantly after treatment of TMP, P<0.01. Conclusion; TMP could reduce the heightened HSP70 and HSP70mRNA expression in cochlear spiral ganglion cells induced by gentamicin to alleviate its oto-toxicity so as to improve hearing function.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2002年第12期929-932,共4页
Chinese Journal of Integrated Traditional and Western Medicine
关键词
川芎嗪注射液
庆大霉素
热休克蛋白
耳蜗
螺旋神经节
ligustrazine injection, gentamicin, heat shock protein, cochlea, spiral ganglion
