摘要
目的 建立流式细胞术非侵入性检测胎儿ABO、Rh(D)血型的实验方法。方法 采用吸收放散试验 ,从人源血清中获得IgG抗 A、抗 B试剂。依据胎儿父母的血型 ,选择IgG的抗 A、抗 B和抗 D作一抗与孕妇外周血红细胞反应 ,以羊抗人IgGF(ab′) 2 FITC作二抗连接A、B、D抗体 ,同时用抗 i PE标记孕妇外周血中的胎儿红细胞。依据所获荧光标记 (FITC和PE)的孕妇和胎儿细胞的散点图格局 ,分析胎儿细胞的ABO和Rh血型。结果 应用所研究的流式细胞检测技术 ,对 6 9名妊娠 8～ 39周的孕妇检测 ,13名孕妇外周血中因未检出胎儿红细胞而无法鉴定 ,其余 5 6名孕妇均检出胎儿细胞 ,她们的胎儿血型产前检测结果与胎儿出生后的检测结果完全吻合。结论 流式细胞术方法能在产前准确和无损伤地检测胎儿血型 。
Objective To establish the FCM method of non invasive detection of fetal ABO and Rh(D) blood groups in maternal blood.Methods Using absorption and elution method, we obtained the IgG anti A and anti B from human sera. The IgG anti A, B, D were used as the first antibody to react with RBCs in maternal peripheral blood.The goat anti human IgG F(ab')2 FITC was used as the second antibody to conjugate anti A, B, D antibodies, Meanwhile anti i PE was used to mark fetal RBCs in maternal peripheral blood.The fluorescence dot plot diagrams of maternal and fetal cells acquired by FCM were used to detect fetal ABO and Rh(D) blood groups.Results Peripheral blood from 69 pregnant women between 8 and 39 weeks of gestation were studied.Fetal cells could not be found in 13 samples.Of the remaining 56 samples,fetal red cells were identified successfully with ABO/Rh(D) blood types identical to those tested after the birth of the baby.Conclusion In women with fetomaternal hemorrhage(FMH) during pregnancy,the FCM method established by the author can accurately and non invasively detect the blood groups of fetuses.This method can possibly be used for diagnosis of hemolytic disease of the newborn.
出处
《中国输血杂志》
CAS
CSCD
2002年第6期378-380,共3页
Chinese Journal of Blood Transfusion
基金
深圳市科技局科研资助课题 (编号 2 0 0 10 40 5 0 )
关键词
胎儿血型
流式细胞术
非侵入性检测
血型鉴定
新生儿溶血病
Fetal ABO and Rh(D) blood groups
Flow cytometry
Non invasive detection
Fetomaternal hemorrhage(FMH)
