摘要
脱脂后的油茶籽粕含有活性成分黄酮醇。通过70%甲醇回流提取,经2 mol/L盐酸水解后分离沉淀,再经丙酮萃取,获得油茶黄酮醇,得率2. 1%,纯度93. 8%。该分离方法简单快速,适合于工业化生产。产物经紫外、质谱、~1H-NMR和^(13)CNMR分析,其结构确认为山柰酚苷元。该产物对DPPH和ABTS自由基的清除能力IC_(50)分别为0. 02%和0. 25%。小鼠灌胃1月后,血清和脑组织中MDA显著降低,SOD和GSH-Px活性显著增高(p<0. 05)。表明该黄酮醇对体内外自由基均有明显的清除效果,对脑损伤有较好保护作用。
Defatted Camellia oleifera seed cake contained active component flavonol,which was extracted by 70%methanol in reflux extraction,and hydrolyzed by 2 mol/L hydrochloric acid,and further extracted from the precipitation by acetone. Its yield was 2. 1%,purity was 93. 8%. The separation process was simple,quick and proper for industrial production. By UV,MS,1 H-NMR and12 C-NMR analysis,the flavonol structure was identified as kaempferol glycoside.Half inhibition concentrations( IC50) of the flavonol on free radicals of DPPH and ABTS were respectively 0. 02% and0. 25%. After 1 month oral administration,MDA in mice serum and brain tissue decreased,SOD and GSH-Px activity increased significantly( p<0. 05). The flavonol could eliminate free radicals in vitro and in vivo,and effectively protect against brain injury.
作者
叶勇
何璇
彭旗
范小娜
张忠杰
张悦
唐洁
YE Yong;HE Xuan;PENG Qi;FAN Xiao-na;ZHANG Zhong-jie;ZHANG Yue;TANG Jie(Gannan Medical University Collaborative Innovation Center for Gannan Oil-tea Camellia Industrial Development,Jiangxi Ganzhou 341000;Department of Pharmaceutical Engineering,School of Chemistry and Chemical Engineering,South China University of Technology,Guangdong Guangzhou 510640,China)
出处
《广州化工》
CAS
2019年第5期55-57,共3页
GuangZhou Chemical Industry
基金
赣南医学院赣南油茶产业开发协同创新中心开放基金资助(No:YP201612)
华南理工大学大学生实践教学基地研究项目(No:Y1162100
Y1171890)
关键词
黄酮醇
自由基
山柰酚
油茶籽粕
flavonol
free radicals
kaempferol
seed cake of Camellia oleifera
