摘要
能源树种小桐子属冷敏植物,受低温胁迫影响涉及到形态、生理、生化、基因表达及调控等多个方面。我们最近通过RNA-Seq技术获得了小桐子低温锻炼转录组(45 251条Unigene)及其数字基因表达谱数据,发现在12℃低温锻炼下有4 185个基因表达量发生变化,其中在不同锻炼时间(12 h,24 h,48 h)内都发生变化的基因有553个。本工作从中选择了22个与对照相比有较大表达差异、新诱导的或被公认的抗冷相关基因,通过半定量RT-PCR及进一步聚类分析检测它们在小桐子不同组织及低温锻炼过程中的表达模式,结合其数字基因表达结果以期找出与小桐子抗冷性密切相关的潜在关键基因,为小桐子抗冷性的遗传改良打下基础。
Jatropha curcas L., a chilling-sensitive energy plant species, suffers from cold stress with responsive changes in many aspects such as morphology, physiology, biochemistry, gene expression and regulation. Recently,we obtained a relatively complete transcriptome(45 251 Unigenes) of chill-hardening J. curcas seedlings and a concomitant digital gene expression profiling data by using RNA-seq approaches, from which a total of 4 185 genes were found to be differentially expressed after 12 h, 24 h, and 48 h cold exposure at 12℃, with 553 common genes in all three treatments. Accordingly, 22 cold tolerance-associated genes, with remarkably differential expression, newly induced or well-recognized, were delicately selected herein and investigated by semi-quantitative RT-PCR and further cluster analyses for their expression patterns. The resultant evidences in combination with the corresponding digital gene expression data, could be used for disclosing those potential key genes for the genetical improvement on the cold tolerance of J. curcas.
出处
《基因组学与应用生物学》
CSCD
北大核心
2014年第6期1196-1205,共10页
Genomics and Applied Biology
基金
国家自然科学基金(31260064
31460059
31460179)
云南省教育厅科研基金重大专项项目(ZD2010004)共同资助
关键词
小桐子
RNA-SEQ
抗冷相关基因
表达模式
聚类分析
Jatropha curcas,RNA-Seq,Cold tolerance-associated gene,Expression pattern,Cluster analysis
