摘要
目的 研究甲泼尼龙对视网膜激光损伤后 Müller细胞胶质纤维酸性蛋白 (glial fibrillaryacidic protein,GFAP)和增生细胞核抗原 (proliferating cell nuclear antigen,PCNA)的影响。 方法 40只Sprague- Danley(SD)大鼠随机分为治疗组和对照组 ,治疗组大鼠在视网膜激光光凝损伤后连续 3 d腹腔注射剂量为 30 mg/ kg的甲泼尼龙 ,对照组大鼠腹腔注射等量生理盐水 ;分别于激光光凝术后 3、7、14、2 8d各处死 5只动物 ,取出眼球 ,用 AFA固定液进行组织固定后做石蜡包埋、切片 ,用免疫组织化学方法测定GFAP和 PCNA的表达。 结果 激光光凝损伤后 3 d ,治疗组和对照组视网膜 Müller细胞均表达PCNA,治疗组 PCNA表达明显较对照组弱 ,3 d后 PCNA表达消失 ;激光光凝损伤后 3 d ,治疗组和对照组视网膜 Müller细胞均表达 GFAP,在各时间点治疗组 GFAP表达明显较对照组弱。 结论 甲泼尼龙可减轻 Müller细胞激光损伤后 PCNA和 GFAP的表达 ,最终影响视网膜激光光凝损伤的瘢痕修复 ,这为研究视网膜激光损伤和防护的机制提供了实验证据。
Objective To investigate the effect of methylprednisolone on the expression of glial fibrillary acidic protein (GFAP) and proliferating cell nuclear antigen (PCNA) in Müller cells of rats' retinae injured by laser. Methods Forty SD rats were randomly divided into two groups and inflicted with laser photocoagulation.The rats in treatment group were given methylprednisolone by intraperitoneal injection with a dose of 30 mg/kg for 3 days.At the 3rd,7th,14th,and 28th day after photocoagulation respectively, the eyes were enucleated,fixed and cut into sections.Immunohistochemical examination was used to detect the expression of PCNA and GFAP. Results After photocoagulation the Müller cells expressed PCNA both in the treatment and control group,and the expression of PCNA decreased sharply after 3 days. The expression of PCNA in treatment group was less than that in control group. After photocoagulation the Müller cells also expressed GFAP and the expression of GFAP lasted for at least 28 days ,and the expression of GFAP expression in the treatment group was less than that in the control group. Conclusion Methylprednisolone can reduce the expression of GFAP and PCNA in Müller cells of rats' retinae injured by laser.
出处
《中华眼底病杂志》
CAS
CSCD
2002年第4期299-301,共3页
Chinese Journal of Ocular Fundus Diseases
基金
全军"十五"医药卫生科研基金资助项目 (0 2 M0 1 4 )
