摘要
目的:将胰管捆扎构建的急性胰腺炎(acute pancreatitis,AP)和慢性胰腺炎(chronic pancreatitis,CP)模型与单纯雨蛙素构建的模型的成模效果进行对比。方法:胰管捆扎术后48 h腹腔注射雨蛙素1次(50μg/kg),术后3 d取材为AP模型,术后21 d取材为CP模型。雨蛙素AP模型通过连续腹腔注射7次雨蛙素(50μg·kg^(-1)·h^(-1))构建,首次注射后24 h取材;雨蛙素CP模型在每周第1、3、6天注射,每天注射6次(50μg·kg^(-1)·h^(-1)),共6周,末次注射后5 d取材。H&E染色用于检测胰腺病理损伤,免疫组化染色用于检测炎症介质和纤维化标志物,Masson染色用于检测胰腺纤维化。结果:与雨蛙素AP模型相比,胰管捆扎AP模型损伤评分更高、炎症介质IL-6、TNF-α表达更高;与雨蛙素CP模型相比,胰管捆扎CP模型中胰腺腺泡细胞缺失程度更重,纤维化占比更大,纤维化标志物α-SMA和CTGF表达更高。结论:与雨蛙素胰腺炎模型相比,胰管捆扎构建的小鼠AP模型具有更重的胰腺损伤,而其构建的CP模型具有更明显的胰腺腺泡缺失和纤维化,总体上,其时间和金钱成本低于雨蛙素模型。
Objective:To compare the pancreatic pathological characteristics of acute pancreatitis(AP)and chronic pancreatitis(CP)models constructed by pancreatic duct ligation with those constructed solely by cerulein.Methods:A single intraperitoneal injection of cerulein(50μg/kg/body weight)was administered 48 h after duct ligation.The mice sacrificed 3 d after ligation were designated as the AP group,while those sacrificed 21 d after ligation were designated as the CP group.The AP model induced by cerulein was established by continuous intraperitoneal injection of cerulein 7 times(50μg·kg^(-1)·h^(-1)),mice were sacrificed 24 h after first injection.The CP model induced by cerulein was established by continuous intraperitoneal injection of cerulein on the 1st,3rd,and 6th day of the week,6 times a day(50μg·kg^(-1)·h^(-1)),for a total of 6 weeks,and mice were sacrificed 5 d after last injection.H&E staining is used to detect the pathological morphology of the pancreas,immunohistochemistry staining is used to detect the expression of inflammatory mediators and fibrosis markers,and Masson staining is used to detect pancreatic fibrosis.Results:Compared to the AP model induced by cerulein,those constructed by ligation of pancreatic duct exhibited a higher patho-logical score and higher expression of inflammatory mediators IL-6 and TNF-α.Compared to the CP model induced by cerulein,those constructed by ligation of pancreatic duct exhibited more obvious loss of pancreatic acinar cells,larger proportion of fibrosis,and higher expression of fibrotic markers includingα-SMA and CTGF.Conclusion:Compared to the pancreatitis model induced by cerulein,pancreatitis model constructed by ligation of pancreatic duct has severer pancreatic damage,while the CP model constructed by it has more obvious pancreatic acinar loss and fibrosis,with lower costs in time and economy than those induced by cerulein.
作者
彭程
涂广平
李志强
PENG Cheng;TU Guangping;LI Zhiqiang(Department of Hepatobiliary and Pancreatic Surgery,Hainan Affiliated Hospital of Hainan Medical University/Hainan Provincial People’s Hospital,Haikou 570311,China;Department of Hepatobiliary and Pancreatic SurgeryⅡ,The Third Xiangya Hospital of Central South University,Changsha 410013,China)
出处
《海南医学院学报》
CAS
北大核心
2024年第24期1841-1847,共7页
Journal of Hainan Medical University
基金
国家自然科学基金青年项目(82100688)。
关键词
急性胰腺炎
慢性胰腺炎
雨蛙素
捆扎
Acute pancreatitis
Chronic pancreatitis
Cerulein
Ligation
