摘要
目的建立11种产地地骨皮指纹图谱,并对地骨皮中地骨皮乙素、地骨皮甲素、东莨菪苷、肉桂酸的含量进行含量测定。方法以Kromasil 100-5C18(4.6 mm×250 mm,5μm)作为该实验的色谱柱;流动相为乙腈(A)-0.3%三氟乙酸水溶液(B)进行梯度洗脱;流速:1.0 mL/min;紫外检测波长:278 nm;柱温:30℃;进样量:10μL。结果20批地骨皮中地骨皮乙素含量范围为(1.5475±1.4817)%,地骨皮甲素含量范围为(0.3737±0.3069)%,东莨菪苷含量范围为(0.0291±0.0247)%,因肉桂酸含量过低且部分产地未检测出肉桂酸,所以不予标注含量范围。11种产地地骨皮共标出8个共有峰,相似度均在0.895以上,通过聚类分析、主成分分析和偏最小二乘判别分析对20批不同来源的地骨皮进行了区分。结论该实验建立的地骨皮指纹图谱分析方法可信度高,方法学考察符合规定。通过指纹图谱与含量测定相结合,应用化学计量分析方法建立了地骨皮质量评价模型,为更好地促进地骨皮在中医临床方面的应用做出了贡献。
Objective To establish the fingerprints of Digupi(Lycii Cortex)from eleven producing areas,and to determine the contents of lycii B,lycii A,scopolin and cinnamic acid.Methods A Kromasil 100-5C18(4.6 mm×250 mm,5μm)column was used as the chromatographic column for this experiment;the mobile phase was acetonitrile(A)-0.3%aqueous trifluoroacetic acid(B)for gradient elution;the flow rate was 1.0 mL/min;the ultraviolet(UV)detection wavelength was 278 nm;the column temperature was 30℃;and the injection volume was 10μL.Results The content ranges of ethyl dermatophyllin(1.5475±1.4817)%,methyldermatophyllin(0.3737±0.3069)%,scopolamine(0.0291±0.0247)%,and the content ranges of cinnamic acid(0.0291±0.0247)%were not marked because of low cinnamic acid content and cinnamic acid was not detected in some of the origins.A total of 8 common peaks were identified in the eleven habitats,and the similarity was above 0.895.After that,cluster analysis,principal component analysis and partial least squares discriminant analysis were carried out on the 20 batches of Digupi(Lycii Cortex)by the peak area of the common peaks.Conclusion The fingerprint analysis method of Digupi(Lycii Cortex)crataegi established in this experiment has high credibility,and the methodological investigation is in accordance with the regulations.Through the combination of fingerprinting and content determination,the chemometric analysis method was applied to establish the quality evaluation model of Digupi(Lycii Cortex),which made a certain contribution to better promote the application of Digupi(Lycii Cortex)in the clinical aspects of traditional Chinese medicine.
作者
庞童奇
单雨薇
李世征
吕延伟
袁媛
PANG Tongqi;SHAN Yuwei;LI Shizheng;LYU Yanwei;YUAN Yuan(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China;Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,Liaoning,China;Center for Innovative Engineering and Technology of Traditional Chinese Medicine,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China)
出处
《辽宁中医药大学学报》
CAS
2025年第1期45-50,共6页
Journal of Liaoning University of Traditional Chinese Medicine
基金
国家科技部重点研发计划项目(2021YFD1600903)
沈阳市中青年高层次创新人才项目(RC180201)
沈阳市科技局科学计划项目(F16-205-1-55)
辽宁名中医工作室项目(2024)。
关键词
地骨皮
指纹图谱
含量测定
化学计量分析
Digupi(Lycii Cortex)
fingerprint
content determination
chemometrics analysis
