摘要
为建立降香黄檀茎段组织培养体系,以‘海林1号’降香黄檀初萌茎段为外植体,研究不同取材时间、基础培养基、消毒方案、外源激素及抗生素添加对茎段诱导、不定芽增殖及瓶苗生根的影响。结果表明:外植体最佳取材时间为4月下旬至5月中旬,最佳消毒方式为1/15 ClO_(2)消毒7 min,以1/2 MS为基础培养基,诱导效果最佳。茎段初代诱导基础培养基激素配方为6-BA 1.50 mg·L^(-1)+NAA 0.20 mg·L^(-1)。培养基中添加抗生素氯霉素100.00 mg·L^(-1)+利福平100.00 mg·L^(-1)时,腋芽诱导率高达到84.24%,污染率仅为13.89%。最适丛生芽增殖配方为1/2 MS+6-BA 2.0 mg·L^(-1)+NAA 0.10 mg·L^(-1),增殖系数为3.33。改良的培养基1/2 MS+6-BA 0.15 mg·L^(-1)+NAA 2.50 mg·L^(-1)为最佳生根培养基,生根率为78.33%。
To establish a tissue culture system for the stem segments of Dalbergia odorifera,the newly sprouted stem segments of D.odorifera‘Hailin No.1’were used as explants to study the effects of different sampling times,basic culture media,disinfection schemes,exogenous hormones,and antibiotic additions on stem segment induction,adventitious shoot proliferation,and bottle seedling rooting.The results showed that,the best time for explants to be taken was from late April to mid May,and the best disinfection method was 1/15 ClO_(2) for 7 min,and 1/2 MS as basic medium.The hormone formula for the first generation induction of stem segments in the basic culture medium was 6-BA 1.50 mg·L-1+NAA 0.20 mg·L-1.When chloramphenicol(100.00 mg·L-1)and rifampicin(100.00 mg·L-1)were added into the medium,the rate of axillary bud induction was 84.24%,and the contamination rate was only 13.89%.The optimal multiplication formula was 1/2 MS+6-BA 2.0 mg·L-1+NAA 0.10 mg·L-1 and the multiplication coefficient was 3.33.The best rooting medium was 1/2 MS+6-BA 0.15 mg·L-1+NAA 2.50 mg·L-1,the rooting rate was 78.33%.
作者
李斌
饶丹丹
王思雅
陈彧
陶昱辰
孟新亚
董晓娜
LI Bin;RAO Dandan;WANG Siya;CHEN Yu;TAO Yuchen;MENG Xinya;DONG Xiaona(Hainan Academy of Forestry,Hainan Academy of Mangrove,Haikou Hainan 571100,China;College of Tropical Agriculture and Forestry,Hainan University/Key Laboratory of Genetics and Germplasm Innovation of Tropical Forest Trees and Ornamental Plants,Ministry of Education/Key Laboratory of Germplasm Resources Biology of Tropical Special Ornamental Plants of Hainan Province,Haikou Hainan 570228,China)
出处
《南方林业科学》
2024年第6期6-12,共7页
South China Forestry Science
基金
海南省省属科研院所技术开发专项(KYYSLK2023-011)
海南省重大科技计划项目(ZDKJ2021031)。
关键词
‘海林1号’降香黄檀
茎段
组织培养
再生体系
Dalbergia odorifera‘Hailin No.1’
stem segment
tissue culture
regeneration system
