摘要
目的 探究杏仁核不同亚区神经元对不同类型的社交刺激的空间响应特征。方法 使用即刻反应蛋白cFos标记策略,结合免疫荧光染色技术,对不同社交组和对照组小鼠社交刺激后的杏仁核进行cFos免疫荧光标记,分析cFos标记神经元的空间表达位置和阳性细胞数量差异,对比分析不同社交组小鼠杏仁核及各亚区神经元的激活水平。结果 社交刺激后与假鼠组相比,攻击鼠(CD1)、雌鼠(Female)、雄鼠(Male)组杏仁核各亚区cFos激活均有增加,而幼鼠刺激组小鼠杏仁核cFos表达未见明显差异。在外侧杏仁核和基底杏仁核亚区,CD1社交刺激组小鼠cFos表达均增加。在腹外侧外侧杏仁核亚区,CD1、Female、Male社交刺激组小鼠cFos表达均增加。在腹内侧外侧杏仁核和基底后外侧杏仁核亚区,CD1、Female社交刺激组小鼠cFos表达增加。在背内侧杏仁核亚区,CD1及Female社交刺激组小鼠cFos表达均增加。结论 小鼠在给予不同社交刺激后其杏仁核不同亚区的神经元激活程度不同。
Objective To explore the spatial responses of neurons in different subregions of amygdala to different types of social stimuli.Methods Immediately reactive protein cFos labeling strategy and immunofluorescence staining technique were used to label the amygdala cFos immunofluorescence of mice in different social groups and control group after social stimulation.By analyzing the differences in the spatial expression position of cFos labeled neurons and the number of positive cells,the activation levels of neurons in amygdala and various subregions of mice in different social groups were compared and analyzed.Results After social stimulation,cFos activation in the amygdala subregions of the CD1,Female and Male groups was increased compared with that of the Fake group,while there was no significant difference in cFos expression in the amygdala of the Pup group.In the lateral amygdala and basal amygdala subregions,cFos expression increased in CD1 social stimulation group.In the ventrolateral part of lateral amygdaloid nucleus subregion,cFos expression increased in CD1,Female and Male social stimulation groups.In the ventromedial part of lateral amygdaloid nucleus and basal posterolateral amygdala subregions,cFos expression increased in CD1 and Female social stimulation groups.In the posterodorsal part of medial amygdaloid nucleus subregion,cFos expression increased in both CD1 and Female social stimulation groups.Conclusion Neurons in different subregions of the amygdala are activated to different degrees after different social stimuli.
作者
袁滋铎
柳俊宇
闫玥辰
白占涛
奚楷文
郭保霖
武胜昔
YUAN Ziduo;LIU Junyu;YAN Yuechen;BAI Zhantao;XI Kaiwen;GUO Baolin;WU Shengxi(Department of Neurobiology,School of Life Sciences,Yan'an University,Yan'an 716099,China;Department of Neurobiology,School of Basic Medical Sciences,Air Force Medical University,Xi'an 710032,China;No.2 Cadet Regiment,School of Basic Medical Sciences,Air Force Medical University,Xi'an 710032,China;No.1 Cadet Regiment,School of Basic Medical Sciences,Air Force Medical University,Xi'an 710032,China)
出处
《空军军医大学学报》
CAS
2024年第12期1376-1381,共6页
Journal of Air Force Medical University
基金
国家自然科学基金青年科学基金(82201699)
陕西省重点研发计划一般项目(2023-YBSF-093)
陕西省自然科学基础研究计划项目(2021JCW-13)
陕西省高校科协青年人才托举计划项目(20220306)。
