摘要
试验旨在从健康驴粪便中分离筛选具有良好益生特性的芽孢杆菌(Bacillus),用于制备驴用微生态制剂。采用形态学观察、生理生化和16S rRNA PCR方法对分离菌株进行鉴定,并对其生物学特性进行初步研究。结果显示,分离到驴源芽孢杆菌12株,经鉴定枯草芽孢杆菌、解淀粉芽孢杆菌各3株,高地芽孢杆菌、短小芽孢杆菌各2株,东京芽孢杆菌、莫海威芽孢杆菌各1株,编号为LV-1~LV-12。5株分离菌在纤维素酶、蛋白酶、淀粉酶选择性培养基上均有水解圈,LV-2产酶性能最好,且对低pH值、胃肠液和胆盐的耐受性优于其他分离株。12株芽孢杆菌分别能够抑制金黄色葡萄球菌(Staphylococcus aureus)、大肠杆菌(Escherichia coli)、沙门氏菌(Salmonella gallinarum)和巴氏杆菌(Pasteurella)生长,且对恩诺沙星、林可霉素、氨曲南和头孢他啶耐药。安全性试验表明,12株芽孢杆菌对小鼠安全,无致病性。研究表明,枯草芽孢杆菌LV-2具有较强产水解酶能力,具有抑制金黄色葡萄球菌和巴氏杆菌特性,抗逆性较好,对多数抗生素敏感且安全无致病性,可作为后续开发驴源益生菌制剂的候选菌种。
The purpose of this experiment was to isolate and screen Bacillus with excellent probiotic properties from healthy donkey feces for the preparation of donkey microecological preparations.The isolated strains were identified by morphological observation,physiological and biochemical tests and 16S rRNA PCR.The biological characteristics of the isolated strains were preliminarily studied.The results showed that 12 strains of Bacillus were isolated and identified,including three strains of Bacillus subtilis,three strains of Bacillus amyloliquefaciens,two strains of Bacillus altitudinis,two strains of Bacillus pumilus,one strain of Bacillus toyonensis and one strain of Bacillus mojavensis,numbered LV-1~LV-12.Five strains of the isolated bacteria had hydrolysis circles on cellulase,protease and amylase selective medium.LV-2 had the best enzyme production performance,and its tolerance to low pH,gastrointestinal fluid and bile salt were better than other isolates.12 strains of Bacillus were able to inhibit the growth of Staphylococcus aureus,Escherichia coli,Salmonella,and Pasteurella,respectively,and were resistant to enrofloxacin,lincomycin,aztreonam and ceftazidime.The security test showed that 12 strains of Bacillus were safe and non-pathogenic to mice.The study indicates that Bacillus subtilis LV-2 has a strong ability to produce hydrolases,has antibacterial activity against Staphylococcus aureus and Pasteurella,has good stress resistance,is sensitive to most antibiotics,and is safe and nonpathogenic.It can be used as a candidate strain for the subsequent development of donkey-derived probiotic preparations.
作者
尹珺伊
刘秋瑾
白长胜
田秋丰
吴宪
陈亮
苗艳
江波涛
YIN Jun-yi;LIU Qiu-jin;BAI Chang-sheng;TIAN Qiu-feng;WU Xian;CHEN Liang;MIAO Yan;JIANG Bo-tao
出处
《饲料研究》
CAS
北大核心
2024年第21期85-92,共8页
Feed Research
基金
黑龙江省农业科学院科技创新跨越工程(项目编号:CX23TS21)
黑龙江省农业科学院畜牧产业创新能力提升项目(项目编号:KJCX202201)。
关键词
驴源
芽孢杆菌
产酶特性
抑菌性
抗生素敏感性
安全性
donkey-derived
Bacillus
enzyme production characteristics
antibacterial
antibiotic sensitivity
security
