摘要
【目的】冬小包脚菇(Volvariella brumalis He sp.nav.)是我国特有的低温草菇品种,探索冬小包脚菇液体菌种发酵培养最优条件,旨在缩减冬小包脚菇菌种生产时间与成本,促进冬小包脚菇标准化、规模化生产。【方法】以冬小包脚菇为研究对象,设置不同碳氮源、温度、pH、接种量水平,对冬小包脚菇液体菌种发酵培养的最优生长条件进行研究,并采用四因素三水平正交试验,得到冬小包脚菇液体菌种发酵培养最佳配方。【结果】冬小包脚菇液体菌种发酵培养最优碳源为葡萄糖,菌丝球干质量为1.35 g/L;最优氮源为发酵豆粕粉,菌丝球干质量为3.76 g/L。选取适宜碳源(葡萄糖、果糖)、氮源(发酵豆粕粉、发酵玉米浆)建立L9(34)正交表进行四因素三水平正交试验,得到发酵培养最优配方为:葡萄糖20.0 g,果糖10.0 g,发酵豆粕粉2.5 g,发酵玉米浆2.5 g,KH_(2)PO_(4)1.0 g,K_(2)HPO_(4)0.5 g,MgSO_(4)·7H_(2)O 0.5 g,V_(B1)10.0 mg,pH值6~7,去离子水1000 mL。采用该配方进行验证,接种量为3%,于18℃、120 r/min条件下培养15 d,菌丝球干质量值达5.79 g/L。采用优化配方探索其他培养条件,得到最适温度为18℃(菌丝球干质量为5.97 g/L)、最适pH值为6~7,且当接种量达7%时,发酵效果最佳(菌丝球干质量为6.31 g/L)。【结论】当接种量为7%时,使用上述优化的液体发酵配方培养冬小包脚菇15 d的菌丝球干质量达6.31 g/L,可直接用于下一步生产,大大缩短冬小包脚菇制种周期。
【Objective】The Volvariella brumalis He sp.nav.is a unique low-temperature mushroom in China.The optimal conditions for the fermentation culture of liquid strains of V.brumalis were investigated,aiming to reduce the production time and costs of V.brumalis strains,and promote the standardized and large-scale production of V.brumalis.【Method】The optimal growth conditions of liquid fermentation culture of V.brumalis were studied from the aspects of carbon sources and nitrogen sources,temperature,pH,and inoculation amount.A four-factor and three-level orthogonal test was conducted to determine the best fermentation formula.【Result】The result showed that the optimal carbon source for liquid fermentation culture of V.brumalis was glucose.And the dry masses of the mycelial bulb was 1.35 g/L.The optimal nitrogen source was fermented soybean meal powder,and the dry masses of the mycelial bulb was 3.76 g/L.Suitable carbon sources(glucose,fructose)and nitrogen sources(fermented soybean meal powder,fermented corn pulp)were selected to establish L9(34)orthogonal table for four-factor and three-level orthogonal tests to obtain the optimal formula for fermentation culture:glucose 20.0 g,fructose 10.0 g,fermented soybean meal powder 2.5 g,fermented corn syrup 2.5 g,KH_(2)PO_(4)1.0 g,K_(2)HPO_(4)0.5 g,MgSO_(4)·7H_(2)O 0.5 g,V_(B1)10.0 mg,pH of 6-7,H_(2)O 1000 mL.The mycelial bulb dry mass reached 5.79 g/L with this formula,and the inoculation amount was 3%when cultured at 18℃and 120 r/min for 15 d.Other culture conditions were explored with an optimized formulation,obtaining an optimum temperature of 18℃,a mycelial bulb dry mass of 5.97 g/L,and an appropriate pH from 6 to 7.And when the inoculation amount reached 7%,the dry mass of the mycelial bulb was 6.31 g/L.【Conclusion】When the inoculation amount was 7%,the dry mass of the mycelial bulb was 6.31 g/L after 15 days of culture,which could be directly used in the next step of production and greatly shortened the seed production cycle of V.brumalis.
作者
熊雪
李鹏
向准
黄静
XIONG Xue;LI Peng;XIANG Zhun;HUANG Jing(Guizhou Institute of Biology,Guiyang 550009,China)
出处
《广东农业科学》
CAS
2024年第10期42-52,共11页
Guangdong Agricultural Sciences
基金
贵州省科技计划项目(黔科合支撑[2021]一般196,黔科合中引地[2024]026)
贵州省食用菌产业技术体系建设功能实验室项目(GZSYJCYJSTX-03)。
关键词
冬小包脚菇
液体发酵
碳源
氮源
正交试验
优化配方
Volvariella brumalis
liquid fermentation
carbon source
nitrogen source
orthogonal test
formula optimization
