摘要
目的使用生物信息学方法鉴定和分析菊花脑WRKY基因家族成员,为后期研究WRKY转录因子调控菊花脑萜烯类物质生物合成提供理论基础。方法利用HMMER、BLAST、Pfam、NCBI等在线工具对菊花脑基因组中的WRKY基因家族成员进行筛选和鉴定;ExPASy、WOLF PSORT等在线工具进行理化性质分析;ClustalW、IQ-Tree、MEME等软件进行系统进化、保守结构域分析;TBtools软件进行染色体定位、串联复制、基因表达模式及可视化分析。结果共鉴定出110个CnaWRKYs,其ORF长度为246-2367 bp,编码蛋白的氨基酸数量为81-788 AA,相对分子质量为9463.98-87062.38 Da,理论等电点为4.63-10.15,93个蛋白预测亚细胞定位于细胞核;系统进化分析将CnaWRKYs蛋白分为3个组,其中Ⅱ组又分为5个亚组;蛋白质结构域丰富,同一组CnaWRKYs蛋白结构域高度保守、保守基序相似;109个菊花脑WRKY基因不均一地分布在9条染色体上,另外有1个分布在Scalfold25上;基因表达热图显示,108个CnaWRKYs基因在整体水平上有表达,WRKY基因家族成员在各个组织中的表达丰度存在明显差异。结论系统分析了菊花脑WRKY基因家族成员,为进一步解析CnaWRKYs基因在菊花脑挥发性芳香化合物萜烯类物质生物合成的作用及其调控机制提供理论依据。
Objective Bioinformatics methods were used to identify and analyze members of the WRKY gene family in Chrysanthemum nankingense,which provided a theoretical basis for further studies on the regulation of WRKY transcription factors on the biosynthesis of terpenes in C.nankingense.Methods Online tools such as HMMER,BLAST,Pfam and NCBI were used to screen and identify the WRKY gene family members in the brain genome of C.nankingense.ExPASy,WOLF PSORT and other online tools for physical and chemical properties analysis;ClustalW,IQTree,MEME and other software for system evolution,conservative domain analysis;TBtools software for chromosome localization,tandem replication,gene expression patterns and visual analysis.Results A total of 110 CnaWRKYs were identified,whose ORF length was 246-2367 bp,amino acid number was 81-788 AA,molecular weight was 9463.98-87062.38 Da,theoretical isoelectric point was 4.63-10.15,and 93 Cnawrkys were predicted to be located in the nucleus.CnaWRKYs protein was divided into 3 groups by phylogenetic analysis,and groupⅡwas divided into 5 subgroups.Protein domains are abundant,and the same group of CnaWRKYs protein domains are highly conserved with similar conserved motifs.There were 109 WRKY genes unevenly distributed on 9 chromosomes,and one WRKY gene was distributed on Scalfold25.The gene expression heat map showed that 108 CnaWRKYs genes were expressed at the whole level,and the expression abundance of WRKY gene family members in different tissues was significantly different.Conclusion The WRKY gene family members of C.nankingense were systematically analyzed to provide theoretical basis for further analysis of the role of CnaWRKYs gene in the biosynthesis of volatile aromatic compounds terpenes in C.nankingense and its regulatory mechanism.
作者
蒋莉萍
徐宏峰
程璐
刘义飞
JIANG Liping;XU Hongfeng;CHENG Lu;LIU Yifei(Wuhan No.1 Hospital,Wuhan 430022,China;College of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430065,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2024年第9期2374-2384,共11页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委员会面上项目(32270231):菊属植物TS/CYP基因簇的起源进化机制及功能研究,负责人:刘义飞。
关键词
菊花脑
WRKY转录因子
次生代谢
串联复制
表达分析
Chrysanthemum nankingense
WRKY transcription factor
Secondary metabolism
Tand-em replication
Expression analysis
