摘要
基于全基因组数据对颠茄WRKY转录因子家族鉴定与表达模式分析,为颠茄WRKY转录因子的生物学功能和作用机制研究提供理论依据。该研究利用生物信息学方法对颠茄WRKY基因家族成员进行鉴定,并对其理化性质、保守基序、启动子顺式作用元件和染色体定位等进行预测,并对光质、温度等环境因子调控下颠茄WRKY基因家族的表达模式进行分析。结果表明从颠茄全基因组数据中共鉴定出28个AbWRKY转录因子随机分布于16条染色体上,编码324~707个氨基酸,AbWRKY蛋白多为酸性不稳定亲水蛋白。根据多序列比对及系统发育分析将WRKY基因家族成员分为2个亚族。保守基序和结构域分析表明,颠茄WRKY转录因子在同一亚族中具有保守的结构特征。通过启动子分析预测,颠茄WRKY家族含有光响应元件、激素响应元件和应激反应响应元件。共线性分析显示AbWRKY24在AbWRKY基因家族的扩增中发挥重要作用。qRT-PCR结果表明,AbWRKY6、AbWRKY8、AbWRKY14和AbWRKY24响应红光胁迫,AbWRKY8、AbWRKY14、AbWRKY24响应黄光/低温复合型胁迫;AbWRKY6和AbWRKY8在叶和茎中显著表达,AbWRKY27和AbWRKY28在须根中显著表达,AbWRKY25在花中显著表达。该研究首次鉴定分析了颠茄WRKY基因家族并分析其在光、温调控下的表达模式,为颠茄WRKY转录因子响应光质、温度环境因子分子机制的深入解析和功能验证奠定研究基础。
Based on whole genome data,the identification and expression pattern analysis of the Atropa belladonna WRKY transcription factor family were conducted to provide a theoretical foundation for studying the biological functions and mechanisms of these transcription factors.In this study,bioinformatics methods were employed to identify members of the A.belladonna WRKY gene family and to predict their physicochemical properties,conserved motifs,promoter cis-acting elements,and chromosomal localization.Additionally,the expression patterns of the A.belladonna WRKY gene family under the regulation of environmental factors such as light quality and temperature were analyzed.The results revealed a total of 28 AbWRKY transcription factors,randomly distributed across 16 chromosomes,encoding 324-707 amino acids.Most AbWRKY proteins were acidic,unstable,and hydrophilic.Based on multiple sequence alignment and phylogenetic analysis,the WRKY gene family members were classified into two subfamilies.Conserved motif and domain analysis indicated that WRKY transcription factors in the same subfamily possessed conserved structural features.Promoter analysis predicted that the A.belladonna WRKY family contained light-responsive elements,hormone-responsive elements,and stress-responsive elements.Collinearity analysis showed that AbWRKY24 plays a crucial role in the expansion of the AbWRKY gene family.Then qRT-PCR results indicated that AbWRKY6,AbWRKY8,AbWRKY14,and AbWRKY24 responded to red light stress,while AbWRKY8,AbWRKY14,and AbWRKY24 responded to yellow light/low-temperature combined stress.AbWRKY6 and AbWRKY8 were significantly expressed in leaves and stems,AbWRKY27 and AbWRKY28 were significantly expressed in fibrous roots,and AbWRKY25 was significantly expressed in flowers.This study is the first to identify and analyze the WRKY gene family in A.belladonna and to examine its expression patterns under light and temperature regulation,laying a foundation for in-depth analysis and functional validation of the molecular mechanisms o
作者
刘汶泽
周省委
张韶珂
王留明
顾旭鹏
初雷霞
乔璐
万婕
张骁
杨林林
董诚明
冯卫生
LIU Wen-ze;ZHOU Sheng-wei;ZHANG Shao-ke;WANG Liu-ming;GU Xu-peng;CHU Lei-xia;QIAO Lu;WAN Jie;ZHANG Xiao;YANG Lin-lin;DONG Cheng-ming;FENG Wei-sheng(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;the First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450046,China;Henan Provincial Ecological Planting Engineering Technology Research Center of Authentic Medicinal Materials,Zhengzhou 450046,China;Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment&Chinese Medicine Development of Henan Province Henan,Zhengzhou 450046,China;Engineering and Technology Center for Chinese Medicine Development of Henan Province,Zhengzhou 450046,China;Xinyang Lingrui Ecological Agriculture Co.,Ltd.,Xinyang 460000,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2024年第21期5843-5855,共13页
China Journal of Chinese Materia Medica
基金
河南省重点研发专项(231111312700,241111310200)
中国博士后科学基金特别资助项目(2014T170252)
国家自然科学基金项目(82104329)
河南省科技攻关项目(242102310549)。
关键词
颠茄
WRKY转录因子
生物信息
表达模式
环境因子
Atropa belladonna
WRKY transcription factor
biological information
expression mode
environmental factor
