摘要
【目的】为鉴定和分析云南栘[木衣](Docynia delavayi)bHLH(DdbHLH)转录因子家族,筛选与果皮花青素生物合成相关的DdbHLH转录因子。【方法】基于云南栘[木衣]红、绿2种颜色果皮的转录组数据,对DdbHLH转录因子家族进行鉴定,并通过生物信息学工具对其bHLH保守结构域、蛋白理化性质、系统进化发育关系以及基因的表达情况进行分析。【结果】在云南栘[木衣]中共鉴定到177个DdbHLH转录因子家族成员,其氨基酸长度为69~699 aa,相对分子质量为7.965~75.419 kDa,平均理论等电点为6.93,大部分属于不稳定亲水蛋白。系统发育进化关系显示,DdbHLH转录因子与拟南芥AtbHLH转录因子共同聚类的有15个亚族,17个DdbHLH转录因子聚类到与花青素相关的第2亚族;结合红、绿果皮中DdbHLH基因表达情况,初步筛选11个在红色果皮中表达量较高的成员,对这11个成员进行差异表达分析,最终获得2个可能参与调控花青素生物合成的DdbHLH基因;再对这2个DdbHLH基因进行RT-qPCR检测,结果显示其相对表达量与转录组测序情况基本一致,证实转录组测序结果的可靠性。【结论】从云南栘[木衣]红、绿果皮转录组数据中鉴定到177个DdbHLH转录因子家族成员,筛选到2个转录因子(DdbHLH68、DdbHLH150)与果皮花青素生物合成相关的成员,为DdbHLH转录因子家族在调控云南栘[木衣]果皮色泽方面的研究奠定基础。
[Objective]The study aimed to identify and analyze the Docynia delavayi bHLH(DdbHLH)transcription factors(TFs)family and to screen for the DdbHLH TFs related to anthocyanin biosynthesis in the pericarp.[Method]In the study,the family of DdbHLH TFs was identified based on transcriptomic data from the red and green pericarp of D.delavayi,and their bHLH conserved structural domains,protein physicochemical properties,phylogenetic relationships,as well as gene expression were analyzed by bioinformatics tools.[Result]The results showed that a total of 177 DdbHLH TFs family members were identified in D.delavayi with amino acids length ranging from 69 to 699 aa,rel-ative molecular weight ranging from about 7.965 to 75.419 kDa,and an average theoretical isoelectric point of 6.93,most of them belonging to unstable hydrophilic proteins.Phylogenetic relationships showed that 15 subfamilies of DdbHLH TFs co-clustered with Arabidopsis AtbHLH TFs and 17 DdbHLH TFs clustered into anthocyanin-related subfamily 2.Combining the expression of DdbHLH genes in red and green peri-carp,ll members with high expression in red pericarp were preliminarily screened,and differential expression analysis was carried out on the 11 members,and 2 DdbHLH genes that may be involved in the regulation of anthocyanin biosynthesis were finally obtained.RT-qPCR results showed that the relative expression levels of these 2 DdbHLH genes were basically consistent with the transcriptome sequencing,which con-firmed the reliability of the high-throughput sequencing results.[Conclusion]In the study,177 members of the DdbHLH TFs family are iden-tified from the transcriptome data of the red and green pericarps of D.delavayi.The two members(DdbHLH68&DdbHLH 150)are screened out and are found to be associated with anthocyanin biosynthesis in the pericarp,which lays a foundation for the study of DdbHLH TFs family in the regulation of the pericarpcolor of D.delavayi.
作者
亚华金
田金红
沈莲文
王大玮
YA Hua-jin;TIAN Jin-hong;SHEN Lian-wen;WANG Da-wei(College of Forestry,Southwest Forestry University/Key Laboratory of Ministry of Education for Conservation and Utilization of Mountain Forest Resources in Southwest China/Key Lab for Forest Genetic and Tree Improvement&Propagation in Universities of Yunnan Province,Kunming 650224,China;Yunnan Institute of Tropical Crops,Xishuangbanna,Yunnan 666100,China)
出处
《西南农业学报》
CSCD
北大核心
2024年第9期2003-2011,共9页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金项目(32060350)
云南省万人计划青年拔尖人才专项(YNWR-QNBJ-2020-230)。
