摘要
目的:探讨绿原酸对氧化损伤细胞的改善作用及相关机制。方法:采用2,2-偶氮二(2-甲基丙基咪)二盐酸盐(2,2’-azobis-2-methyl-propanimidamide,AAPH)诱导体外培养的人胚肾细胞建立氧化损伤模型,并给予绿原酸进行干预,测定细胞中谷胱甘肽过氧化物酶活力、丙二醛含量、超氧化物歧化酶活性等抗氧化指标以及氧化损伤相关通路蛋白的表达。结果:绿原酸可降低活性氧水平和丙二醛含量(p<0.05),提高谷胱甘肽含量、谷胱甘肽过氧化物酶活力、过氧化氢酶活力以及超氧化物歧化酶活性(p<0.05),可通过调控Kelch样环氧氯丙烷相关蛋白1/血红素氧合酶1(Kelch-like ECH-associated protein 1/Heme Oxygenase 1,Keap1/HO-1)通路以及降低NADPH氧化酶4(NADPH Oxidase 4,NOX4)的水平(p<0.05)来改善细胞氧化损伤情况。结论:绿原酸的抗氧化机理可能与通过对Keap1/HO-1通路和NOX4蛋白的调控相关。
Objective:To study the effect of improvement and related mechanism of chlorogenic acid on oxidation-damaged cells.Method:Human embryonic kidney 293 cells(HEK293 cells)cultured in vitro were induced by 2,2’-azobis-2-methyl-prolimidamide(AAPH)to establish the oxidative damage model,and chlorogenic acid was given to intervention.Antioxidant indexes such as glutathione peroxidase activity,malondialdehyde content,superoxide dismutase activity and the expression of oxidative damage related pathway proteins in the cells were determined.Result:Reactive oxygen species levels and malondialdehyde content were reduced by chlorogenic acid(p<0.05).Glutathione content,glutathione peroxidase activity,catalase activity and superoxide dismutase activity were incerased by chlorogenic acid(p<0.05).And chlorogenic acid can improve cellular oxidative damage by regulating the Kelch-like ECH-associated protein 1/heme oxygenase 1(Keap1/HO-1)pathway and reducing the expression of NADPH oxidase 4(NOX4)(p<0.05).Conclusion:The antioxidant mechanism of chlorogenic acid may be related to its regulation of the Keap1/HO-1 pathway and NOX4 protein.
作者
黄平情
HUANG Pingqing(Nanhua Hospital Affiliated to Nanhua University,Hengyang 421200,China)
出处
《食品安全导刊》
2024年第34期82-89,共8页
China Food Safety Magazine
